Research Article | Volume: 5, Issue: 12, December, 2015

Optimization of Fermentation Medium for the Production of Recombinant Human Antithrombin III from Saccharomyces cerevisiae through Statistical Experimental Designs

Maheswara Reddy Mallu Sandeep Vemula Srinivasa Reddy Ronda   

Open Access   

Published:  Dec 27, 2015

DOI: 10.7324/JAPS.2015.501203

This work, for the first time reports the optimization of recombinant human antithrombin (rhAT) production in Saccharomyces cerevisiae BY4741 using statistical experimental designs. Most applications of design of experiments (DoE) have concerned optimization of the composition of growth and production culture media. The typical objective is to identify a best selection and quantitative composition of significant medium supplements. In the present study, Plackett-Burman (PB) design followed by central composite design has been employed to evaluate and optimize the suitable culture medium for rhAT production. Influence of raffinose, glutamic acid and vitamin mixture were screened to be significant variables by PB design. The significant nutritional variables were further optimized using central composite design (CCD) for maximum production of rhAT. Central composite design (CCD) has been selected to explain the interaction effect of the three significant variables such as raffinose, glutamic acid, and vitamin mixture. The multiple regression equation (R2=0.9967) was used to optimize significant impact of medium component values to maximize rhAT formation. The optimized values were found to be 23.09765 g/L, 8.01816 g/L and 77.2056 mg/L for raffinose, glutamic acid and vitamin mixture, respectively. The maximum yield of rhAT of 38.97 µg/mL was obtained experimentally using CCD and was very close to the predicted response of 38.93 µg/mL.

Keyword:     Optimization Placket-Barman design Central composite design Antithrombin Saccharomyces cerevisiae.


Maheswara Reddy Mallu, Sandeep Vemula, Srinivasa Reddy Ronda. Optimization of Fermentation Medium for the Production of Recombinant Antihrombin III from Saccharomyces cerevisiae through Statistical Experimental Designs. J App Pharm Sci, 2015; 5 (12): 014-020.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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