Research Article | Volume : 1 Issue : 7, September 2011

Antioxidant and antihyperglycemic activities of methanolic extract of Glinus oppositifolius leaves

Nazia Hoque Mohammad Zafar Imam Saleha Akter Md. Ehsanul Hoque Mazumder S.M. Raquibul Hasan Jamiuddin Ahmed Md. Sohel Rana   

Open Access   

Abstract

The present study was carried out to investigate the antioxidant and antidiabetic potential of methanolic extract of Glinus oppositifolius leaves. The antioxidant potential was examined by DPPH radical scavenging assay, nitric oxide (NO) scavenging assay, determination of total flavonoid content and total antioxidant capacity assay. Antihyperglycemic activity was studied by oral glucose tolerance test (OGTT) in normal mice and in alloxan-induced diabetic mice. In DPPH radical scavenging assay the IC50 value of the extract was found to be greater than 1000 µg/ml (ascorbic acid, IC50 = 14.45 µg/ml) while it was 269 µg/ml in nitric oxide (NO) scavenging assay (quercetin IC50 = 15.24 µg/ml). The amount of total flavonoid was 25 .46 mg/g and total antioxidant activity was 79.48 mg/g equivalent to Quercetin and Ascorbic acid respectively. A significant effect (p<0.05) on oral glucose tolerance was noted at the dose of 200 mg/kg and 400 mg/kg body weight in mice. At the same doses, the extract showed significant (p<0.05) reduction of blood glucose level in Alloxan-induced diabetic mice compared to the standard drug Metformin. Overall, the results of the present study indicate that the methanolic extract of Glinus oppositifolius leaves possess moderate antioxidant activity and significant antihyperglycemic activity.


Keyword:     Glinus oppositifolius antioxidant antihyperglycemic alloxan oral glucose tolerance.


Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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