Research Article | Volume: 5, Issue: 10, October, 2015

Phenylalanine enhances Quercetin content in In vitro cultures of Abutilon indicum L.

Ramgopal Rao Sajjalaguddam Anitha Paladugu   

Open Access   

Published:  Oct 28, 2015

DOI: 10.7324/JAPS.2015.501014
Abstract

Flavonoids have aroused considerable interest because of their potential beneficial effect on human health. Quercetin is one of the most abundant natural flavonoids present in medicinal plants. In the present study, a comparative phytochemical analysis of bioactive compounds from leaf and leaf derived callus of Abutilon indicum L. was carried out. Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) analysis of methanolic extract of Abutilon leaf revealed the presence of quercetin. To test the effect of precursor in enhancing flavonoid content, in vitro studies were conducted by supplementing phenylalanine to actively growing callus cultures of Abutilon. More than threefold increase in quercetin content was obtained in elicitor induced callus compared to control.


Keyword:     Abutilon indicum Callus Flavonoids Quercetin HPLC Elicitor Phenylalanine.


Citation:

Ramgopal Rao Sajjalaguddam, Anitha Paladugu. Phenylalanine enhances Quercetin content in In vitro cultures of Abutilon indicum L. J App Pharm Sci, 2015; 5 (10): 080-084.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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