Research Article | Volume: 5, Issue: 10, October, 2015

Variation in the marker content of five different Dendrobium species: Comparative evaluation using validated HPTLC technique

Sunita Shailajan Suman Kumaria Deepti Gurjar Mayuresh Joshi Prasenjit Paul Nasheman Khongthaw   

Open Access   

Published:  Oct 28, 2015

DOI: 10.7324/JAPS.2015.501006

Northeast India represents a rich floristic wealth of India. Orchids form a very noticeable feature of this vegetation. Amongst orchids, the genus Dendrobium forms a very important group of plants which are used by the locals for the treatment of various ailments. A through literature survey revealed that there is a paucity of data on the phytochemical evaluation of Dendrobium species using analytical methods. The chromatographic technique such as HPTLC is reported to be useful for standardization of plant materials in terms of phytochemical markers and evaluation of their quality. Therefore, in this study, five different Dendrobium species have been evaluated and compared in terms of their marker content using validated HPTLC methods. All the samples analyzed were found to be a good source of β-sitosterol with maximum content in D. fimbriatum stem and minimum content in D. chrysanthum roots. D. nobile (roots and stem) and D. moschatum (stem) were also found to be a source of ursolic acid and lupeol, respectively. Thus, this work will contribute in the identification, quality evaluation and standardization of different Dendrobium species. On the basis of maximum bioactive marker content, specific part of Dendrobium species can be selected which may be supported by its efficacy.

Keyword:     Dendrobium species HPTLC phenolics phytochemical variation terpenoids.


Sunita Shailajan, Suman Kumaria, Deepti Gurjar, Mayuresh Joshi, Prasenjit Paul, Nasheman Khongthaw. Variation in the marker content of five different Dendrobium species: Comparative evaluation using validated HPTLC technique. J App Pharm Sci, 2015; 5 (10): 032-038.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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