Research Article | Volume: 5, Issue: 8, August, 2015

2-Methoxy-1,4-Naphthoquinone (MNQ) Suppresses Protein Kinase C ßI, δ, and ζ Expression in Raji Cells

Wong Teck Yew,  Liew Kitson,  Anthony Ho Siong Hock,  Gan Seng Chiew,  Lim Yang Mooi   
Wong Teck Yew1, Liew Kitson2, Anthony Ho Siong Hock2, Gan Seng Chiew1, Lim Yang Mooi1

1Department of Pre-Clinical Sciences, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Lot PT 21144. Jalan Sungai Long, Bandar Sungai Long, 43000 Kajang, Selangor, Malaysia.

2School of Biosciences, Taylor’s University, Lakeside Campus 1, Jalan Taylor's, 47500 Subang Jaya, Selangor, Malaysia.

Open Access   

Published:  Aug 28, 2015

DOI: 10.7324/JAPS.2015.50801
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Abstract

Protein Kinase C (PKC) is widely documented to be involved in the regulation of cancer cell growth, proliferation, survival, inflammation and apoptosis. This study evaluates the capability of 2-Methoxy-1,4-Naphthoquinone (MNQ) in regulating PMA-induced PKC expression in human Burkitt’s lymphoma cell line (Raji cells). MNQ exhibited stronger anti-tumour-promoting activity than genistein based on the inhibitory assay of Epstein-Barr virus (EBV) activation. The IC50 values attained were 2.92 and 7.40 μM, respectively. The suppressive effects of MNQ on PKC expression was determined by using the PepTag® non-radioactive detection of PKC assay. The IC50 values achieved for staurosporine and rottlerin (standard control), and MNQ were 0.01, 6.38, and 13.13 μM, respectively. These preliminary results indicate that MNQ specifically suppressed the expression of PKC βI, δ, and ζ in a concentration-dependent manner in Raji cells.


Keyword:     Protein kinase C Anti-tumour-promoting 2-Methoxy-14-Naphthoquinone Cancer chemoprevention

Citation:

Wong Teck Yew, Liew Kitson, Anthony Ho Siong Hock, Gan Seng Chiew, Lim Yang Mooi. 2-Methoxy-1,4-Naphthoquinone (MNQ) Suppresses Protein Kinase C βI, δ, and ζ Expression in Raji Cells. J App Pharm Sci, 2015; 5 (08): 001-005.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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