Research Article | Volume: 5, Issue: 7, July, 2015

Broad spectrum antibacterial activity of granaticinic acid, isolated from Streptomyces thermoviolaceus NT1; an endophyte in Catharanthus roseus (L.) G. Don

Sudipta RoyDebdulal Banerjee   

Open Access   

Published:  Jul 27, 2015

DOI: 10.7324/JAPS.2015.50702
Abstract

Searching for endophytic actinomycetes, strain NT1 was isolated from surface sterilized stem of Catharanthus roseus (L.) G. Don collected from Paschim Medinipur, India. The strain was identified as Streptomyces thermoviolaceus NT1 on the basis of morphological, biochemical and 16s rDNA based phylogenetic analysis. It showed potential antagonism against several Gram positive and Gram negative bacterial pathogens along with drug resistant Staphylococcus aureus. Maximum antibacterial production was obtained in ISP2 media at pH 7.2, 35 °C for 10 days. The active antibacterial substance was purified by Silica gel column chromatography and activity guided TLC. IR and NMR analysis identified the active compound as granaticinic acid of m/z 463.26 [M+H]. These results suggest that the antimicrobial produced by the isolated endophytic strain will be useful in near future.


Keyword:     EndophyteStreptomyces thermoviolaceusgranaticinic acidantibacterialdrug resistance.


Citation:

Sudipta Roy, Debdulal Banerjee. Broad spectrum antibacterial activity of granaticinic acid, isolated from Streptomyces thermoviolaceus NT1; an endophyte in Catharanthus roseus (L.) G. Don. J App Pharm Sci, 2015; 5 (07): 006-011.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Article Metrics
366 Views 48 Downloads 414 Total

Year

Month

Related Search

By author names