Research Article | Volume: 5, Issue: 6, June, 2015

A Simplified Liquid Chromatography-Mass Spectrometry Method for Simultaneous Determination of Pyrimethamine, Sulphadoxine and Artesunate in Human Plasma

S M Sandhya P S Shijikumar   

Open Access   

Published:  Jun 27, 2015

DOI: 10.7324/JAPS.2015.50618
Abstract

This paper presents the development of a new LC-MS method for the determination of pyrimethamine, sulphadoxine and artesunate in human plasma. The analytes were extracted by liquid-liquid extraction with ethyl acetate. The analytes and internal standard artemether were separated on a Phenomenex C18 column using gradient elution with 20mM ammonium acetate (mobile phase A) and methanol (mobile phase B) both containing 0.5% acetic acid at a flow rate of 0.8 ml/min. The calibration curves were constructed over the range of 5-30 ng/ml for pyrimethamine, 50-300 ng/ml for sulphadoxine and 25-150 ng/ml for artesunate respectively. Within day and between-day precision and accuracy did not exceed 8%. All the three analytes were found to be stable in plasma samples with no evidence of degradation during three freeze-thaw cycles and three months storage in -20 °C. According to the validation results, the proposed method was found to be specific, accurate, precise and could be applied to the simultaneous quantitative analysis of pyrimethamine, sulphadoxine and artesunate in human plasma.


Keyword:     Pyrimethamine Sulphadoxine Artesunate Artemether LC-MS Plasma Validation.


Citation:

Sandhya S M, Shiji kumar PS. A Simplified Liquid Chromatography-Mass Spectrometry Method for Simultaneous Determination of Pyrimethamine, Sulphadoxine and Artesunate in Human Plasma. J App Pharm Sci, 2015; 5 (06): 109-114.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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