Short Communication | Volume: 5, Issue: 3, March, 2015

Visible spectro-photometric determination of cerberin in rat plasma

S. S. Prasanth A. Rajasekaran   

Open Access   

Published:  Mar 28, 2015

DOI: 10.7324/JAPS.2015.50319

The present paper aims to develop a simple direct colorimetric method for the determination of cerberin in rat plasma without any previous chemical separation of the cerberin from Cerbera odollam. The method is based on reaction of the cardenolide group of cerberin with 3,5-dinitro salicylic acid [DNS] in alkaline medium which yields a bright orange-yellow complex that exhibits absorption maxima at 370nm.Beer’s law obeyed in the concentration range of 50-250µg/mL. The result of the method was validated statistically and by recovery studies.

Keyword:     Cerberin Biological fluid Visible Spectrometry.


Prasanth SS, Rajasekaran A. Visible spectrophotometric determination of cerberin in rat plasma. J App Pharm Sci, 2015; 5 (03): 109-112.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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