Research Article | Volume: 4; Issue: 11, November, 2014

Acute Toxicity and Suppressive Effects of a Curcumin Analogue Gamavuton-0 (Gvt-0) On CFA-Induced Arthritis in rats

Zullies Ikawati Nunung Yuniarti Supardjan Amir Margono   

Open Access   

Published:  Nov 27, 2014

DOI: 10.7324/JAPS.2014.4114

Gamavuton-0 (GVT-0) is a curcumin analogue, which is synthesized from acetone and vanillin with chloride acid as a catalyst and ethanol as a solvent. The compound has been reported to have an anti-inflammatory effect related to its activity as COX-2 inhibitor, anti-oxydant, and radical scavanger. This study was aimed to investigate whether the GVT-0 has a suppressive effect on rheumatoid arthritis (RA) as one of chronic inflammatory disorders in a rat model. Wistar rats were immunized with Complete Freund’s Adjuvant (CFA). After a second CFA immunization, the rats were treated with GVT-0 orally at 10, 20, 40, 80 mg/kg BW once a day for 21 days, while the positive control received methotrexate 0.22 mg/kg BW. The animal paws were evaluated macroscopically for redness, swelling and deformities with Smit method to assess arthritic index. The anti-inflammatory effect of GVT-0 was evaluated using a plethismograph by measuring rat paw edema, while it’s effects on the level of TNF- and IL-1β in the ankle joints were examined using an ELISA method. The effect of GVT-0 on cartilage destruction was assesed histologically using Safranin-O staining. The acute toxicity test was also performed to assess the safety potential of the compound. The oral treatment of rats for 21 days with various doses of GVT-0 significantly suppressed the progession of RA indicated by the improvement of arthritic index and decreased the inflammation in rat paws. The compound also decreased the level of TNFα and IL-1β in ankle joints. The destruction of cartilage was significantly reduced in rats ankles after treatment with GVT-0. In toxicological assay, the apparent LD50 value of GT-0 was regarded as 7,29 g/kg BW and was classiefied to be practically non-toxic. The results suggest that GVT-0 is safe and potential to modify the progression of rheumatoid arthritis and can be developed as a new disease modifying anti rheumatoid arthritis drugs (DMARDs).

Keyword:     Gamavuton-0 curcumin analogue rheumatoid arthritis acute toxicity


Zullies Ikawati, Nunung Yuniarti, Supardjan Amir Margono. Acute Toxicity and Suppressive Effects of a Curcumin Analogue Gamavuton-0 (Gvt-0) On Cfa-Induced Arthritis in rats. J App Pharm Sci, 2014; 4 (11): 019-023.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text


Article Metrics
614 Views 30 Downloads 644 Total



Related Search

By author names