Research Article | Volume: 4; Issue: 9, September, 2014

In Vitro α-Amylase Inhibition and Antioxidant potential of Chloroform Fraction of Hydroalcoholic Extract Obtained from Hyptis Suaveolens

P. Nayak D.M. Kar S. Nayak   

Open Access   

Published:  Sep 27, 2014

DOI: 10.7324/JAPS.2014.40908
Abstract

The plant Hyptis is an effective medicinal herb and a well known medicinal plant in herbal world. The present study was aimed to investigate the α-amylase inhibition and antioxidant activities of chloroform fraction of H. suaveolens. Chloroform fraction of H. suaveolens was screened for α-amylase inhibition activity by 3, 5-dinitrosalicylic acid (DNSA) method respectively. and antioxidant activity was evaluated by 1,1-diphenyl-2-picryl-hydrazile (DPPH) free radical scavenging, Super oxide radical Scavenging, nitric oxide (NO) radical scavenging, and 2.2’-azinobis-3-ethylbenzothiazole-6-sulfonic acid (ABTS) radical scavenging assays. The chloroform fraction of H. suaveolens showed effective α-amylase inhibition activity (IC50 57.34µg/ml). Chloroform fraction demonstrated significant antioxidant activity in all the in vitro antioxidant models. Moreover, the chloroform fraction was found to be extremely effective in scavenging DPPH (IC50 57.51 µg/ml) while compared to super oxide (IC50 61.36 µg/ml), NO (IC5076.3 µg/ml) and ABTS radical (IC50 93.16 µg/ml) scavenging activity. In conclusion, from the results of present study it is established that antioxidant and alpha-amylase inhibitory activity of chloroform fraction of H. suaveolens may contribute in its earlier observed antidiabetic potential.


Keyword:     Alpha-amylase inhibitory activity H. suaveolens DPPH Super oxide radical Scavenging activity.


Citation:

Shweta P Nayak, Durga M Kar. In Vitro α-Amylase Inhibition and Antioxidant potential of Chloroform Fraction of Hydroalcoholic Extract Obtained from Hyptis Suaveolens. App Pharm Sci, 2014; 4 (09): 046-051.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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