Pongamia pinnata L. (fabaceae) is a small evergreen tree reported in traditional literature as ‘Karanj’. Seeds of this plant have been used in the management of skin diseases, ulcer, piles, bronchitis; leprosy etc. Seeds are reported to possess phytoconstituents like pongapin, pongamol, pongaglabrone, kanjone, karanjin etc. The therapeutic activities of seeds are mainly attributed to its major furanoflavone karanjin. Thus, in the present research work the pharmacokinetics of karanjin was determined in Sprague dawley female rats using a validated RP-HPLC method from the ethanolic extract of P. pinnata (p.o.). The pharmacokinetic parameters were calculated using non compartmental model of analysis. Findings of the present work would be applicable for the future investigation on P. pinnata and karanjin for their different therapeutic uses and would be useful for justifying their dosage and route of administration from their allied formulations.