Detection of uronic acid of Lactobacillus rhamnosus PN04

Lactobacillus rhamnosus is known as species that had many benefits in pharmaceutical, dairy food industry. To supply more applications for life, the detection of uronic production of Lactobacillus rhamnosus PN04 was done. After the extracellular components of Lactobacillus rhamnosus PN04 culture were precipitated with 1% cetyl pyridium chloride, the precipitants were used to check the uronic acid production capacity by carbazole assay according to BP98. As a result, the highest uronic acid yield was produced at 96 h cultivation. The maximum of uronic acid was about 74mg/L. The study was the first report the uronic acid existing in Lactobacillus rhamnosus PN04.