Research Article | Volume: 3, Issue: 12, December, 2013

Studies Involving a Commercial Extract of Three Ballerina: ii- Evaluation of the in Vitro Effect on the Labeling of Blood Constituents of rats with Technetium-99m and on the Morphology of the Red Blood Cell

Nelson de Souza Pinto,  Lúcia de Fátima Amorim,  Dulciane Nunes Paiva,  Vilma Gomes Barreto,  Gláucio Diré ,  Sebastião David Santos-Filho,  Mario Bernardo-Filho   
Nelson de Souza Pinto1, Lúcia de Fátima Amorim1, Dulciane Nunes Paiva2, Vilma Gomes Barreto3, Gláucio Diré4 & 5, Sebastião David Santos-Filho6, Mario Bernardo-Filho6,7

1Programa de Pós-Graduação em Ciências da Saúde da Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil

2Universidade de Santa Cruz do Sul, Santa Cruz do Sul, RS, Brasil;

3Universidade Federal da Bahia, Salvador, BH, Brasil;

4Universidade Estadual da Zona Oeste, Rio de Janeiro, RJ, Brasil;

5Universidade Estácio de Sá, Rio de Janeiro, RJ, Brasil;

6Laboratório de Radiofarmácia Experimental, Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil;

7Coordenadoria de Pesquisa, Instituto Nacional do Câncer, Rio de Janeiro, RJ, Brasil.

Open Access   

Published:  Dec 30, 2013

DOI: 10.7324/JAPS.2013.31215
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Abstract

The aim of this work is to characterize (electric conductivity and refractive index) a Three Ballerina (TB) and to evaluate its in vitro effect on the labeling of blood constituents with 99mTc and on the morphology of the red blood cells (RBC). Anticoagulated whole blood (Wistar rat) was incubated with a TB extract and the labeling of the blood constituents with technetium-99m (99mTc) was performed. Plasma (P) and blood cells (BC) were isolated and aliquots were also precipitated with trichloroacetic acid to separate soluble (SF) and insoluble fractions (IF). The %ATI in these samples was calculated. The morphology of the treated RBC showed no shape’s qualitative alterations. The TB extract was characterized with an electric conductivity of 1.35±0.04mSv/cm and refractive index of 2.21±0.15%BRIX. TB extract decreased significantly (p<0.05) the radioactivity distribution in the cellular compartment from 96.97±1.30% to 88.48±7.13%, and in IF-P from 74.29±4.12 to 14.26±5.73%. In conclusion, our data show some physical chemical parameters that could be suitable to characterize the preparation of an extract of TB. Moreover, substances present in the TB extract should probably have an effect on transport of the ions through the RBC membrane and/or should have redoxi properties and the stannous ion would decrease and could justify the effect on the fixation of the radioactivity on the plasma proteins. Moreover, although our experiments were carried out with animals, it is suggested precaution in the interpretation of the examinations that use labeled blood constituents in patients who are undergone TB extract.


Keyword:     Blood constituents technetium-99m red blood cell Three ballerina electric conductivity refractive index.

Citation:

Nelson de Souza Pinto, Lúcia de Fátima Amorim, Dulciane Nunes Paiva, Vilma Gomes Barreto, Gláucio Diré, Sebastião David Santos-Filho, Mario Bernardo-Filho. Studies Involving a Commercial Extract of Three Ballerina: ii- Evaluation of the in Vitro Effect on the Labeling of Blood Constituents of rats with Technetium-99m and on the Morphology of the Red Blood Cell. J App Pharm Sci, 2013; 3 (12): 083-088.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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