Research Article | Volume: 3, Issue: 10, October, 2013

Studies Involving a Commercial Extract of three Ballerina: I- Characterization and the Effect on the Binding of two 99mtechnetium-Radiopharmaceuticals on Blood Constituents

Nelson de Souza Pinto Lúcia de Fátima Amorim Dulciane Nunes Paiva Vilma Gomes Barreto Gláucio Diré Feliciano Sebastião David Santos-Filho Mario Bernardo-Filho   

Open Access   

Published:  Oct 31, 2013

DOI: 10.7324/JAPS.2013.31019
Abstract

We have determined some physicochemical parameters of an aqueous extract of Three ballerina (3TB) and its influence on the binding of the radiopharmaceuticals sodium pertechnetate (Na99mTcO4) and 99mTc-methylene diphosphonate (MDP-99mTc) on blood constituents. Whole blood of rats was incubated with a 3TB extract and with Na99mTcO4 or MDP-99mTc. Plasma (P) and blood cells (BC) from Wistar rats (control and treated) were separated. P and BC were precipitated with trichloroacetic acid (TCA) and soluble (SF) and insoluble fractions (IF) isolated. The percentage of incorporated radioactivity (%ATI) in each fraction was determined. The treatment not influenced statistically (p>0.05) the %ATI of both radiopharmaceuticals on BC and P compartments, as well as on IF-P and SF-P isolated by TCA precipitation. However, 3TB has altered the fixation of the 99mTc-MDP on the IF-BC and SF-BC. We are firstly reported physicochemical parameters (absorption spectrum, viscosimetry and pHmetry) about an aqueous 3TB extract. Moreover, it is possible to speculate that an alteration would be not found on the biodistribution of the 99mTc-pertechnetate in the person that is undergoing 3TB extract. However, this would be found with the 99mTc-MDP.


Keyword:     Binding blood three ballerina sodium pertechnetate methylene diphosphonate proteins.


Citation:

Nelson de Souza Pinto, Lúcia de Fátima Amorim, Dulciane Nunes Paiva, Vilma Gomes Barreto, Gláucio Diré, Sebastião David Santos-Filho, Mario Bernardo-Filho. Studies Involving a Commercial Extract of three Ballerina: I- Characterization and the Effect on the Binding of two 99mtechnetium-Radiopharmaceuticals on Blood Constituents. J App Pharm Sci, 2013; 3 (10): 109-113.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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