Research Article | Volume: 3, Issue: 11, November, 2013

Evaluation of intraperitoneal ozone application effects to rat’s antioxidant enzymes, superoxide dismutase and glutathione peroxidase

Yannis V Simos,  Ioannis K Toliopoulos , Ioannis I Verginadis,  Ilias V Karagounis,  Pavlos S Charalampidis ,  Vasilios N Ragos,  Angelos M Evangelou,  Spiros C Karkabounas   
Yannis V Simos1, Ioannis K Toliopoulos1 & 2,Ioannis I Verginadis1, Ilias V Karagounis1, Pavlos S Charalampidis1 & 3, Vasilios N Ragos4, Angelos M Evangelou1, Spiros C Karkabounas1

1Laboratory of Physiology, Medical School, University of Ioannina, Ioannina, Greece.

2Konstantinion Research Center of Molecular Medicine and Biotechnology, non-profit foundation, Thessaloniki, Greece.

3Strimonikos Health Center, Serres, Greece. 4Maxillofacial Surgery, Faculty of Medicine, University of Ioannina, 45110 Ioannina, Greece.

Open Access   

Published:  Nov 29, 2013

DOI: 10.7324/JAPS.2013.31107
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Abstract

Ozone is a powerful oxidant that presents dual activity: either as a therapeutic or as a toxic agent. The aim of this study was to investigate the effects of intraperitoneal (i.p.) application of ozone on the endogenous antioxidant enzymes of Wistar rats compared with the amendment of the total antioxidant capacity in blood and urine. Rats were divided randomly into two groups: the control group (CG) which was injected with placebo (NaCl 0.9%) and the ozone group (OG) which was administrated daily for 10 consecutive days with ozone at a concentration of 6.3μg/Kg body weight. Blood and urine samples were collected at the beginning, after the administration period and 22 days after the initiation of the experiment. Intraperitoneal application of ozone leads to a potent Increase to glutathione peroxidase (GPx) levels with no effect to Superoxide dismutase (SOD) levels. Total antioxidant capacity (TAC) was significantly decreased in plasma, red blood cells (RBCs) and urine of the rats. Malondialdehyde (MDA) concentration in urine remained stable. To our knowledge these are the first experimental results showing that repeated i.p. application of ozone in low doses produce severe changes to the whole antioxidant capacity of the blood and indications of toxicity, as the organism exert efforts to adapt to oxidative stress.


Keyword:     Ozone oxidative stress antioxidant enzymes total antioxidant capacity.

Citation:

Yannis V Simos, Ioannis K Toliopoulos, Ioannis I Verginadis, Ilias V Karagounis, Pavlos S Charalampidis, Vasilios N Ragos, Angelos M Evangelou, Spiros C Karkabounas. Evaluation of intraperitoneal ozone application effects to rat’s antioxidant enzymes, superoxide dismutase and glutathione peroxidase. J App Pharm Sci, 2013; 3 (11): 035-039.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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