Research Article | Volume: 3, Issue: 7, July, 2013
Research Article | Volume: 3, Issue: 7, July, 2013
The objective of this study was to evaluate the utility of derivative spectrophotometric method for analysis of Diclofenac sodium (DIC) and Thiocolchicoside (THIO) in combination. Derivative spectrophotometry has allowed specific determination of Diclofenac sodium at 249 nm with negligible contribution by Thiocolchicoside. Similarly, Thiocolchicoside was determined at 246nm with negligible interference by Diclofenac sodium. The described method obeyed Beer’s law in the range of and 4-36μg/ml for DIC 2-10μg/ml for THIO. Validation parameters such as linearity, accuracy, precision, specificity, and LOD and LOQ values were performed. The percentage recovery was 99.52% for DIC and 99.32% for THIO. Intra and Interday precision %RSD values were <2. Thus from the results obtained it can be concluded that proposed method is simple, rapid and specific.
Vishruti Choksi, Daxa Vasava, Rajeshree Chaudhari, Bhavna Patel, Shraddha Parmar., Method Development and Validation of Second Order Derivative Spectrophotometric Method for Simultaneous Estimation of Diclofenac Sodium and Thiocolchicoside from its Pharmaceutical Formulation. J App Pharm Sci, 2013; 3 (07): 097- 100.
Year
Month