Research Article | Volume: 3, Issue: 7, July, 2013

Method Development and Validation of Second Order Derivative Spectrophotometric Method for Simultaneous Estimation of Diclofenac Sodium and Thiocolchicoside from its Pharmaceutical Formulation

Vishruti Choksi Daxa Vasava Rajeshree Chaudhari Bhavna Patel Shraddha Parmar   

Open Access   

Published:  Jul 30, 2013

DOI: 10.7324/JAPS.2013.3718
Abstract

The objective of this study was to evaluate the utility of derivative spectrophotometric method for analysis of Diclofenac sodium (DIC) and Thiocolchicoside (THIO) in combination. Derivative spectrophotometry has allowed specific determination of Diclofenac sodium at 249 nm with negligible contribution by Thiocolchicoside. Similarly, Thiocolchicoside was determined at 246nm with negligible interference by Diclofenac sodium. The described method obeyed Beer’s law in the range of and 4-36μg/ml for DIC 2-10μg/ml for THIO. Validation parameters such as linearity, accuracy, precision, specificity, and LOD and LOQ values were performed. The percentage recovery was 99.52% for DIC and 99.32% for THIO. Intra and Interday precision %RSD values were <2. Thus from the results obtained it can be concluded that proposed method is simple, rapid and specific.


Keyword:     Diclofenac sodium (DIC) Thiocolchicoside (THIO) De


Citation:

Vishruti Choksi, Daxa Vasava, Rajeshree Chaudhari, Bhavna Patel, Shraddha Parmar., Method Development and Validation of Second Order Derivative Spectrophotometric Method for Simultaneous Estimation of Diclofenac Sodium and Thiocolchicoside from its Pharmaceutical Formulation. J App Pharm Sci, 2013; 3 (07): 097- 100.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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