Published:  Jul 30, 2013DOI: 10.7324/JAPS.2013.3708
The objective of the work was to establish a new, rapid and sensitive HPLC–DAD method for simultaneous determination of three most commonly prescribed drugs; hyoscine, ketoprofen, and ibuprofen. The HPLC separation of the analytes was performed on a hypersil - Gold C18 (150 mm × 4.6 mm, 8 μm) column, using gradient elution of the mobile phase composed of 0.01 M potassium phosphate dibasic containing 2 g/L heptane sulphonic acid sodium salt maintained at pH 3.5 (Pump A) and acetonitrile 80% v/v (pump B) with a flow rate of 2 mL/min. The multiple wavelength detector was set at 210 nm for measurement of all compounds. Quantification was based on measuring the peak areas. The three compounds were resolved with retention times 6.42 ± 0.009, 10.63 ± 0.006 and 16.43 ± 0.008 min for hyoscine, ketoprofen and ibuprofen, respectively.The calibration curves were linear in the range of 0.64 – 96, 0.64 – 400 and 1.28 – 640 µg/mL for hyoscine, ketoprofen and ibuprofen, respectively, all of them with coefficients of determination above 0.9995. The methodology recoveries were higher than 95.0%. The limits of detection (LODs) were 0.11, 0.17 and 0.17 µg/mL for hyoscine, ketoprofen, and ibuprofen, respectively. The intra- and inter-day coefficients of variation were less than 2%. The method is accurate, sensitive and simple for quality control as well as for stability indicating purposes.
Rasha A. Shaalan, Rim S. Haggag, Saeid F. Belal and Mahmoud Agami., Simultaneous Determination of Hyoscine, Ketoprofen and Ibuprofen in Pharmaceutical Formulations by HPLC - DAD. J App Pharm Sci, 2013; 3 (07): 038-047.
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