Research Article | Volume: 3, Issue: 6, June, 2013

An approach on microbial biosynthesis of L-glutaminase: a tumour inhibitor

Sunil Dutt P.L.N.S.N. Siddalingeshwara K.G Karthic J Mohsin S. Mushtaq Naveen M Vishwanatha T and Prathiba K.S   

Open Access   

Published:  Jun 27, 2013

DOI: 10.7324/JAPS.2013.3622

L-Glutaminase, an amidohydrolase enzyme has been a choice of interest in the treatment of lymphoblastic leukemia. This study investigates the production of extracellular L-glutaminase synthesis were carried out by using Aspergillus oryzae was evaluated under different fermentation parameters by employing submerged fermentation method. The L-glutaminase producers detected by the pink zone around the colony by simple plate assay method. Aspergillus oryzae S2 is the potential strain among the fungal isolates. The L-glutaminase synthesis were increased their yield after the optimization of fermentation parameters. The optimum pH 5.0, temperature 350C and inoculum size 1.0 ml and it showed 217.65 IU.

Keyword:     L-glutaminase fermentation kinetics antitumour age


Sunil Dutt P L N S N , Siddalingeshwara K.G, Karthic J, Mohsin S. Mushtaq, Naveen M Vishwanatha T and Prathiba K.S., An Approach on Microbial Biosynthesis of L-Glutaminase – A Tumour Inhibitor. J App Pharm Sci, 2013; 3 (06): 132-135.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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