Research Article | Volume: 3, Issue: 5, May, 2013

Optimized Ultrasonic-Assisted Extraction of Polysaccharides from Chinese Traditional Medicinal Plant Paris fargesii Using Orthogonal Design

Gang Xiang Dan Wang Gui-Hua Zou Xing Huang Meng-Liang Tian and Feng Gao   

Open Access   

Published:  May 30, 2013

DOI: 10.7324/JAPS.2013.3521

Paris fargesii is a famous Chinese traditional medicinal plant used as antipyretic, antidotal, antiphlogistic and analgesic. This research aimed at the determination of the total polysaccharides contents in P. fargesii, which were collected from Baoxing country of Sichuan Province in order to get the optimized extraction technology. Ultrasonic method was adopted in the extraction of total polysaccharides from P. fargesii. Through single factor experiment and orthogonal test, the effects of ultrasonic extraction conditions on the total polysaccharides extraction were measured, including solid-liquid ratio, ultrasonic temperature, ultrasonic power and ultrasonic time. Then colorimetric method was applied for the assaying. The optimized extraction conditions of total polysaccharides from P. fargesii were as follows: ultrasonic power: 600w, solid-liquid ratio: 1:20, ultrasonic extraction time: 1.5h and ultrasonic temperature: 90℃. Under the optimum parameters, the total polysaccharides extraction efficiency was 0.0715%.

Keyword:     Paris fargesii total polysaccharides ultrasonic-assisted extraction orthogonal design.


Gang Xiang, Dan Wang, Gui-Hua Zou, Xing Huang, Meng-Liang Tian, and Feng Gao. Evaluating the incidence, risk factors and glycaemic control of new-onset diabetes mellitus in kidney transplant recipients: a single centre study. J App Pharm Sci. 2013; 3 (05): 110-113.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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