Research Article | Volume: 3, Issue: 5, May, 2013

RP-HPLC Method Development and Validation of Gallic acid in Polyherbal Tablet Formulation

Kamal Kardani Nilesh Gurav Bhavna Solanki Prateek Patel Bhavna Patel   

Open Access   

Published:  May 30, 2013

DOI: 10.7324/JAPS.2013.3508
Abstract

A new simple, accurate, precise, sensitive and validated RP-HPLC method was developed for the estimation of Gallic acid in bulk and pharmaceutical tablet dosage form. The chromatographic conditions used for the separation was Phenomenex Luna C18 (2) (4.6 x 250mm, 5μ), rheodyne manual injector with capacity of 20µL and mobile phase comprised of Water: Acetonitrile (80: 20%v/v) and pH is maintained at 3.00 using O-phosphoric acid (OPA). The flow rate was 1.0mL/min with detection at 272nm. The retention time was found to be 3.60min. The linearity was found to be in the range of 0.5-50µg/mL for Gallic acid with correlation coefficient of 0.9994. The proposed method is accurate with 99.97% - 100.58 % recovery and precise (%RSD of repeatability, intra-day and inter-day variations were 1.26%, 0.48-0.95%, 0.80-1.83%). The Limit of Detection (LOD) and Limit of Quantification (LOQ) were found to be 0.0178µg/mL and 0.0539 µg/mL respectively. The amount of Gallic acid in Polyherbal tablet was found to be 1.63%.


Keyword:     Gallic acid RP-HPLC Validation Polyherbal formula


Citation:

Kamal Kardani, Nilesh Gurav, Bhavna Solanki, Prateek Patel, Bhavna Patel., RP-HPLC Method Development and Validation of Gallic acid in Polyherbal Tablet Formulation. J App Pharm Sci, 2013; 3 (05): 037-042.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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