Research Article | Volume: 3, Issue: 4, April, 2013

Rapid high Performance liquid Chromatography- Tandem mass Spectrometry Method For Quantitation of Milnacepran in Human Plasma

Umesh Thorat   

Open Access   

Published:  Apr 27, 2013

DOI: 10.7324/JAPS.2013.3427
Abstract

A highly sensitive, rapid and specific liquid chromatography–mass spectrometric (LC-MS/MS) method for the determination of milnacepran in human plasma using anastrazole as the internal standard (I.S.) was developed and validated. The sample work-up procedure involved a liquid–liquid extraction of the compounds. The chromatographic separation was performed on a Thermo Hypersil–Hypurity C18 column (50 × 4.6 mm, 5 μm). Mass spectrometric data were acquired in MRM monitoring of the [M + H] + ions at 246.80 > 100.10 and 294.10 > 224.90 for milnacipran and anastrazole, respectively. The method was validated in the concentration range of 2–1,000 ng mL−1 (r2 > 0.99). The method was validated and found suitable to high-throughput analysis of human plasma samples for application in pharmacokinetic and bioavailability/bioequivalance studies.


Keyword:     Milnacepran Human plasma LC–MS/MS Pharmacok


Citation:

Umesh Thorat, Snehal Gomes, Jagdhish Baheti., Rapid high Performance liquid Chromatography- Tandem mass Spectrometry Method For Quantitation of Milnacepran in Human Plasma. J App Pharm Sci, 2013; 3 (04): 146-151.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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