Research Article | Volume: 3, Issue: 4, April, 2013

A RP-HPLC Method Development and Validation for the Estimation of Gliclazide in bulk and Pharmaceutical Dosage Forms

B.V.V Ravi kumar A.K. Patnaik Saroj Kumar Raul Nagireddy Neelakanta Rao   

Open Access   

Published:  Apr 27, 2013

DOI: 10.7324/JAPS.2013.3410
Abstract

A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Gliclazide in pharmaceutical dosage form. Isocratic elution at a flow rate of 1.2 ml min -1 was employed on a symmetry C18 column at ambient temperature. The mobile phase consisted of methanol: phosphate buffer 50:50 (V/V). The UV detection wavelength was at 210 nm. Linearity was observed in concentration range of 1-100 µg/mL. The retention time for Gliclazide was 3.25 min. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of Gliclazide in pharmaceutical dosage forms.


Keyword:     Gliclazide RP-HPLC Method development Validation.


Citation:

B.V.V Ravi kumar, A.K. Patnaik, Saroj Kumar Raul, Nagireddy Neelakanta Rao, A RP-HPLC Method Development and Validation for the Estimation of Gliclazide in bulk and Pharmaceutical Dosage Forms. J App Pharm Sci, 2013; 3 (04): 059-062.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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