Research Article | Volume: 3, Issue: 2, February, 2013

Screening and characterization of protease-producing Virgibacillus, Halobacillus and Oceanobacillus strains from Thai fermented fish

Thanapun Taprig Ancharida Akaracharanya Jaruwan Sitdhipol Wonnop Visessanguan and Somboon Tanasupawat   

Open Access   

Published:  Feb 27, 2013

DOI: 10.7324/JAPS.2013.30205
Abstract

Halophilic bacteria from fermented fish (pla-ra and pla-chom), shrimp paste (ka-pi) and fermented crab were isolated and screened for protease activities. Twenty-seven isolates exhibited protease specific activities ranged from 0.12 to 3.62 unit/mg protein. Isolate TPPN2-1 from pla-ra produced maximal protease specific activity (3.62 units/mg protein). They were Gram-positive, moderately halophilic rod-shaped bacteria and were divided into 5 groups on the basis of their phenotypic and chemotaxonomic characteristics, DNA-DNA relatedness including the 16S rRNA gene sequences analyses. Three isolates (Group I) were identified as Virgibacillus halodenitrificans, 3 isolates (Group II) as V. marismortui, 7 isolates (Group III) as V. dokdonensis, 10 isolates (Group IVA & IVB) as Halobacillus species and 4 isolates (Group V) as Oceanobacillus iheyensis.


Keyword:     Fermented fish Virgibacillus &l


Citation:

Thanapun Taprig, Ancharida Akaracharanya, Jaruwan Sitdhipol, Wonnop Visessanguan and Somboon Tanasupawat., Screening and characterization of protease-producing Virgibacillus, Halobacillus and Oceanobacillus strains from Thai fermented fish. J App Pharm Sci. 2013; 3 (02): 025-030.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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