Research Article | Volume: 3, Issue: 2, February, 2013

Screening and characterization of protease-producing Virgibacillus, Halobacillus and Oceanobacillus strains from Thai fermented fish

Thanapun Taprig,  Ancharida Akaracharanya,  Jaruwan Sitdhipol,  Wonnop Visessanguan and Somboon Tanasupawat   
Thanapun Taprig1, Ancharida Akaracharanya1, Jaruwan Sitdhipol2, Wonnop Visessanguan3 and Somboon Tanasupawat4

1Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.

2Bioscience Department, Thailand Institute of Scientific and Technological Research, Pathum Thani, Thailand.

3Food Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani, Thailand.

4Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.

Open Access   

Published:  Feb 27, 2013

DOI: 10.7324/JAPS.2013.30205
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Abstract

Halophilic bacteria from fermented fish (pla-ra and pla-chom), shrimp paste (ka-pi) and fermented crab were isolated and screened for protease activities. Twenty-seven isolates exhibited protease specific activities ranged from 0.12 to 3.62 unit/mg protein. Isolate TPPN2-1 from pla-ra produced maximal protease specific activity (3.62 units/mg protein). They were Gram-positive, moderately halophilic rod-shaped bacteria and were divided into 5 groups on the basis of their phenotypic and chemotaxonomic characteristics, DNA-DNA relatedness including the 16S rRNA gene sequences analyses. Three isolates (Group I) were identified as Virgibacillus halodenitrificans, 3 isolates (Group II) as V. marismortui, 7 isolates (Group III) as V. dokdonensis, 10 isolates (Group IVA & IVB) as Halobacillus species and 4 isolates (Group V) as Oceanobacillus iheyensis.


Keyword:     Fermented fish Virgibacillus &l

Citation:

Thanapun Taprig, Ancharida Akaracharanya, Jaruwan Sitdhipol, Wonnop Visessanguan and Somboon Tanasupawat., Screening and characterization of protease-producing Virgibacillus, Halobacillus and Oceanobacillus strains from Thai fermented fish. J App Pharm Sci. 2013; 3 (02): 025-030.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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