3',4'-Dimethoxy Quercetin, a Flavonol Compound Isolated from Kalanchoe pinnata

From methanol extract of Kalanchoe pinnata (Lam.) Pers. leaves, a flavonol compound has been isolated. Fractionation of the methanol extract with ethyl acetate, followed by ethyl acetate fraction purification using column chromatography method with ethyl acetate : n-hexane as mobile phase gave a yellow crystal compound (A). Further analysis using 1D- and 2D-nuclear magnetic resonance (NMR), confirmed with mass spectrometer (LC-MS), compound A identified as 3',4'-dimethoxy quercetin.