Research Article | Volume: 3, Issue: 1, January, 2013

Optimization of Nutritive Factors in Culture Media for Growth and Tropane Alkaloid Production from Anisodus acutangulus Hairy Roots

Qijia Liu Lijie Cui Yingying Guo Xiaoling Ni Yan Zhang Guoyin Kai   

Open Access   

Published:  Jan 28, 2013

DOI: 10.7324/JAPS.2013.30101

Anisodus acutangulus hairy roots were grown in N6 medium which was optimal for growth and alkaloid production. The cell biomass and alkaloid yield reached 2.7 g l-1 and 3.9 mg l-1 (dry weight) respectively in sucrose medium, higher than those obtained in other carbon source media. 90 mM nitrogen (NH4+/NO3- = 4:1) gave the highest cell yield (4.5 g l-1) and the maximum alkaloid production (9.9 mg l-1). The cell yield (4.1 g l-1) of hairy roots grown at pH 6.5 was 2 times higher than that at pH 4.5. However, the maximum alkaloid production (7.2 mg l-1) was yielded at pH 4.5.

Keyword:     Alkaloid production Anisodus acutangulusHairy root


Qijia Liu. Lijie Cui. Yingying Guo Xiaoling Ni. Yan Zhang. Guoyin Kai., Optimization Of Nutritive Factors In Culture Media For Growth And Tropane Alkaloid Production From Anisodus Acutangulus Hairy Roots. J App Pharm Sci. 2013; 3 (01): 001-004.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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