Research Article | Volume: 3, Issue: 1, January, 2013

Optimization of Nutritive Factors in Culture Media for Growth and Tropane Alkaloid Production from Anisodus acutangulus Hairy Roots

Qijia Liu,  Lijie Cui,  Yingying Guo,  Xiaoling Ni,  Yan Zhang,  Guoyin Kai   
Qijia Liu1, Lijie Cui1, Yingying Guo1, Xiaoling Ni2, Yan Zhang1, Guoyin Kai1

1Plant Germplasm Resources Research and Development Center, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, P. R. China.

2Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China

Open Access   

Published:  Jan 28, 2013

DOI: 10.7324/JAPS.2013.30101
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Abstract

Anisodus acutangulus hairy roots were grown in N6 medium which was optimal for growth and alkaloid production. The cell biomass and alkaloid yield reached 2.7 g l-1 and 3.9 mg l-1 (dry weight) respectively in sucrose medium, higher than those obtained in other carbon source media. 90 mM nitrogen (NH4+/NO3- = 4:1) gave the highest cell yield (4.5 g l-1) and the maximum alkaloid production (9.9 mg l-1). The cell yield (4.1 g l-1) of hairy roots grown at pH 6.5 was 2 times higher than that at pH 4.5. However, the maximum alkaloid production (7.2 mg l-1) was yielded at pH 4.5.


Keyword:     Alkaloid production Anisodus acutangulusHairy root

Citation:

Qijia Liu. Lijie Cui. Yingying Guo Xiaoling Ni. Yan Zhang. Guoyin Kai., Optimization Of Nutritive Factors In Culture Media For Growth And Tropane Alkaloid Production From Anisodus Acutangulus Hairy Roots. J App Pharm Sci. 2013; 3 (01): 001-004.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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