Research Article | Volume: 2 Issue: 12, December, 2012

Validation of DNA Damage Progression with Days after Single Exposure of Sublethal Dosage of Electron Beam Radiation

Madhu L N Suchetha Kumari N Vijay R   

Open Access    DOI: 10.7324/JAPS.2012.21205


The aim of this study was to asses the DNA damage progression with days after single exposure of 6Gy electron beam radiation (EBR). Molecular DNA damage in lymphocytes of mice was assessed using alkaline comet assay and in bone marrow cells by micronucleus assay. In comet assay %DNA in tail, Comet length, Olive Tail Moment (OTM) served as a measure of DNA damage and in micronucleus test, frequency of micronucleus formation in bone marrow cells was measured to evaluate the DNA damage. The experiment was performed by taking 24 healthy Swiss albino mice with body weight 30+5g. The animals were grouped into four. Group1 served as control, Group 2, 3 and 4 were irradiated by 6Gy EBR. Animals of Group 2, 3 and 4 were sacrificed on 5th, 10th and 15th day post irradiation respectively. The comet assay procedure was carried out for all these groups by using lymphocytes separated from EDTA treated blood. The micronucleus test was performed in bone marrow cells (PCE-Poly chromatic erythrocytes, NCE-normochromatic erythrocytes). The slides prepared for this were scored for the measure of DNA damage. The results showed all comet parameter were significantly (P<0.05) affected by prolonging the post irradiation days from zero (control) to 5, 10 and 15. There was an alteration found in the PCE/PCE+NCE ratio in irradiated mice. A linear increase in the micronucleus formation was observed in post irradiated days. These results conclude a progression in DNA damage with days, post irradiation.

Keyword:     Electron Beam Radiation DNA damage Micronucleus Comet.


Madhu L N, Suchetha Kumari N, Vijay R., Validation of DNA Damage Progression with Days after Single Exposure of Sublethal Dosage of Electron Beam Radiation. J App Pharm Sci. 2012; 2 (12): 023-026.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text


Article Metrics

677 Absract views 6 PDF Downloads 683 Total views

   Abstract      Pdf Download

Related Search

By author names

Citiaion Alert By Google Scholar

Name Required
Email Required Invalid Email Address

Comment required