Research Article | Volume: 2 Issue: 10, October, 2012

Antibacterial and antifungal activities of Actinobacteria isolated from Rathnagiri hills

S. Silambarasan E. Praveen kumar T. Murugan D. Saravanan and R. Balagurunathan   

Open Access    DOI: 10.7324/JAPS.2012.21020


This study was performed to isolate actinomycete colonies having antibacterial and antifungal activity from soil samples. A total of 27 actinomycete colonies were isolated in pure culture from five soil samples using Starch casein agar medium. Entire isolates were screened for their antimicrobial activity by agar plug method against five each of human pathogenic bacteria and fungi. Of this, 7 strains inhibits B. substilis, 3 strains inhibits Klebseilla sp, 6 strains inhibits B. cerus, 5 strains inhibits S. aureus and only 2 strains inhibits E. coli. Incase of fungi all the actinobateria has moderate activity with less fungal strains, only 1 strain (RA 5) inhibits entire fungus except Penicillium sp. The metabolites from potent strain was produced by fermentation, separated by centrifugation, it was tested for their antimicrobial activity against the test bacterial and fungal strains by well diffusion and disc diffusion method. In this study, the metabolites from RA5 (identified as Streptomyces sp.) have showed good antibacterial and antifungal activity. Since many isolates showed inhibitory activity against indicator bacteria, it is suggestive that Rathnagiri hill’s soil could be an interesting source to explore for antibacterial secondary metabolites.

Keyword:     AntinomycetesSecondary metabolitesAntimicrobial activityStreptomyces sp.


S. Silambarasan, E. Praveen kumar, T. Murugan, D. Saravanan and R. Balagurunathan. Antibacterial and antifungal activities of Actinobacteria isolated from Rathnagiri hills. J App Pharm Sci. 2012; 2 (10): 099-103.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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