Research Article | Volume: 2 Issue: 10, October, 2012

Phytochemical Analysis and Antiviral Potential of Aqueous Leaf Extract of Psidium guajava Against Newcastle Disease Virus in ovo

Chollom S.C., Agada G.O.A., Bot D.Y.,  Okolo M.O,  Dantong D.D., Choji T. P.,  Echeonwu B. C.,  Bigwan E.I., Lokason S and Banwat E.   
1 & 4Chollom S.C.,1Agada G.O.A.,1Bot D.Y., 4Okolo M.O, 2Dantong D.D.,1Choji T. P., 3Echeonwu B. C., 5Bigwan E.I.,1Lokason S and 4Banwat E.

1National Veterinary Research Institute, Vom, Plateau State, Nigeria.

2Faculty of Veterinary Medicine, University of Abuja, Abuja, Nigeria.

3Federal College of Veterinary and Medical Laboratory Technology, Vom, Plateau State, Nigeria.

4Department of Medical Microbiology, University of Jos, Plateau State, Nigeria.

5Department of Medical Laboratory Science University of Jos, Plateau State, Nigeria.

Open Access    DOI: 10.7324/JAPS.2012.21009

PDF [ 0.1 MB]
Request Permission
Share
Download Citation
Print
Download PDF
Abstract

Leaf extract of Psidium guajava were subjected to phytochemical screening and in ovo antiviral assay against Newcastle Disease Virus (NDV). Phytochemical analysis revealed the presence of pharmacologically active and nutritionally relevant compounds. Nine-day-old embryonated chicken eggs were divided into ten groups of fives and received various treatments. Groups 1-6 received 100EID50/0.1ml NDV pre-treated with P. guajava leaf extracts at final concentrations of 250, 200, 100, 50, 25 and 10mg/ml in that order. Controls were included. Embryo survival was observed daily. Allantoic fluid from treated eggs and serum from hatched chicks were collected for spot hemagglutination (HA) and hemagglutination inhibition (HI) tests to detect NDV in the eggs and antibodies against NDV in the hatched chicks respectively. Results showed that embryo survival was higher with higher extract concentrations.. Just as increase in extract concentration was directly proportional to virus death and inversely proportional to production of antibody against NDV in hatched chicks. The current findings have clearly demonstrated that P. guajava leaf extract has nutritional value as well as great antiviral potential against NDV in ovo. In vivo trials are needed to validate the use of the tree in controlling Newcastle disease in chickens.


Keyword:     Phytochemical ScreeningNewcastle Disease VirusPsidium guajavaIn ovo.

Citation:

Chollom S.C., Agada G.O.A., Bot D.Y., Okolo M.O, Dantong D.D., Choji T. P., Echeonwu B. C., Bigwan E.I., Lokason S and Banwat E. Phytochemical Analysis and Antiviral Potential of Aqueous Leaf Extract of Psidium guajava Against Newcastle Disease Virus in ovo. J App Pharm Sci. 2012; 2 (10): 045-049.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
HTML Full Text

Reference

Article Metrics
381 Views 33 Downloads 414 Total

Year

Month

Related Search

By author names