Research Article | Volume: 2 Issue: 10, October, 2012

Study of the Pepsin Enzymatic Activity in in-vitro Dissolution Test of Bromazepam Tablets by UV/VIS Spectrophotometry

Guilherme Nobre Lima do NascimentoEurico Viegas Montalvao and Tales Alexandre Aversi-Ferreira   

Open Access    DOI: 10.7324/JAPS.2012.21002

Abstract

The in vitro dissolution test is an essential parameter to determine the properties of biopharmaceutical formulations. Therefore, the purpose of this study was to evaluate the dissolution of solid Bromazepam formulations and different enzyme concentrations. The dissolution test for Bromazepam tablets was performed using a Vankel VK 7000, apparatus 2 (paddle), at temperature of 37.0°C. The dissolution medium simulated gastric fluid with pepsin, which was prepared with 2 g of sodium chloride, varying concentrations of purified pepsin and 7 mL of hydrochloric acid (PA) in 1000 mL of water. The pH was maintained close to 1.2. The results obtained in the dissolution test showed that the samples have been influenced by the presence of pepsin for both similar drug and reference drug. Among the various enzyme concentrations tested, the highest enzyme concentration tested showed the best results. Thus, it could be concluded that the increase in enzyme concentration increases the dissolution of both drugs, causing a decrease in the test time.




Citation:

Guilherme Nobre Lima do Nascimento, Eurico Viegas Montalvão, Tales Alexandre Aversi-Ferreira, Study of the Pepsin Enzymatic Activity in in vitro Dissolution Test of Bromazepam Tablets by UV/VIS Spectrophotometry. J App Pharm Sci. 2012; 2 (10): 011- 015.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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