Comparative study was carried out on the production of protease using isolate from moraine. The isolates were screened on gelatin plates for gelatin hydrolyses by the different isolates. Among the all tested isolate No. 7 EHN produced the highest activity. While under the fermentation conditions after 3 days the maximum yield of protease was 277 U/ml. The protease production from marine isolate could be commercially used for applications purpose.