Research Article | Volume: 2 Issue: 7, July 2012

Supporting the immune system through functional modulation of carbon tetrachloride intoxicated splenic macrophages by administering Tinospora cordifolia

Biswajit Chakraborty Mahuya Sengupta   

Open Access    DOI: 10.7324/JAPS.2012.2715


The suppression of immune system leads to the development of several chronic illnesses; in replying to this the available allopathic system of medicine is provided with limited tools for treatment and prevention. In this context, the present investigation focuses on immunostimulant properties of an aqueous extract of Tinospora cordifolia in splenic macrophages isolated from carbon tetrachloride induced immunosuppressive mice. Our results suggested that carbon tetra chloride intoxication (0.5ml/Kg body weight) significantly altered the functional status like morphology, cell adhesion, phagocytosis, myeloperoxidase (MPO) release, nitric oxide (NO) release, DNA fragmentation and killing capacity of splenic macrophages. However administration of aqueous fraction of Tinospora cordifolia stem parts at a dose of 40 mg/kg body weight (in vivo) in CCl4 intoxicated male albino mice ameliorated the effect of CCl4, as was evident from the scanning electron micrographs, percentage of morphologically altered macrophages, phagocytosis activity, cell adhesion, MPO release, NO release, DNA fragmentation and intracellular killing capacity. Thus, it has been coclude that bioactive compounds presents in polar fractions of Tinospora cordifolia were accountable for boosting the immune system.

Keyword:     Splenic macrophages Tinospora cordifolia aqueous extract CCl4 intoxication immunostimulant.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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