Research Article | Volume: 2 Issue: 7, July 2012

Development and Validation of RP-HPLC-PDA Method for Simultaneous Estimation of Baclofenand Tizanidine in Bulk and Dosage Forms

Buchi N. Nalluri K. Sushmitha B. Sunandana D. Prasad Babu   

Open Access    DOI: 10.7324/JAPS.2012.2714

Abstract

A simple, specific, and accurate reverse phase liquid chromatographic method was developed for the estimation of Tizanidine (TZN) and Baclofen (BCF) in combination. A Phenomenex -C18 (150×4.60 mm Dimensions, 5μm Particle size) column with mobile phase containing methanol: water (53:47) was used at isocratic mode and eluents were monitored at228 nm. The retention times of TZN and BCF were 2.03 and 4.1min respectively and both the drugs showed good linearity in the concentration range of 10-50 μg/mL with a correlation coefficient (R) of 0.9992 and 0.9993 respectively. The proposed method was validated as per ICH guidelines and method showed good precision with percent relative standard deviation less than 2%. The percentage assay values of TZN and BCF were found to be 99.72 and 98.56respectively and recovery values are within the limits of 98-102% indicating the proposed method was accurate and precise for the simultaneous estimation of TZN and BCF in bulk and pharmaceutical dosage forms.


Keyword:     Simultaneous estimationReverse phase liquid chromatographyValidation.


Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Article Metrics
450 Views 40 Downloads 490 Total

Year

Month

Related Search

By author names