Evaluation of the herbal formulation HPH-031508PP in mitigating complications associated with dengue in experimental studies

Mohammed Mukhram Azeemuddin Madan Mittlekoppa Narendra Onkaramurthy Mallappa Raghavendra Pralhada Rao Bindu Indira Mohamed Rafiq Hucharayappa Ganesh   

Open Access   

Published:  Aug 16, 2025

DOI: 10.7324/JAPS.2025.227453
Abstract

Dengue is a mosquito-borne viral infection characterized by symptoms and pathological conditions such as fever, thrombocytopenia, vascular leakage, and hepatic damage. A comprehensive and holistic approach is essential to manage these conditions associated with dengue effectively. The objective of this study was to assess the efficacy of HPH-031508PP, an herbal formulation, in the treatment of complications associated with dengue. This study was performed on Wistar rats. Cyclophosphamide was used to induce thrombocytopenia in rats; lipopolysaccharide was used to induce microvascular leakage; pyrexia was induced using Brewer’s yeast; and hepatic damage was induced using carbon tetrachloride. The rats were administered HPH-031508PP at 250 and 500 mg/kg of body weight. Treatment with HPH-031508PP helped in increasing platelet count (reversing thrombocytopenia); reducing peripheral platelet destruction; enhancing the host immune response; protecting vital organs by decreasing microvascular permeability; safeguarding hepatic function; and regulating fever. The study results showed that HPH-031508PP has the potential to ameliorate complications associated with dengue. HPH-031508PP, a polyherbal formulation, comprises hydroalcoholic extracts of Carica papaya L. leaves, Phyllanthus maderaspatensis L. whole plant, Tinospora cordifolia stem, and Piper nigrum L. fruit. Herbal ingredients present in HPH-031508PP synergistically exhibit antiplatelet, anti-dengue, antipyretic, antiviral, hepatoprotective, and anti-inflammatory properties, which are in turn needed to treat complications associated with dengue.


Keyword:     Carica papaya L. thrombocytopenia microvascular leakage dengue


Citation:

Azeemuddin MM, Narendra MM, Mallappa O, Rao RP, Indira B, Rafiq M, Ganesh H. Evaluation of the herbal formulation HPH-031508PP in mitigating complications associated with dengue in experimental studies. J Appl Pharm Sci. 2025. Article in Press. http://doi.org/10.7324/JAPS.2025.247748

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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