Scrophularia buergeriana Miq., belonging to the family Scophulariaceae, is a potential medicinal herb that has been used in traditional oriental medicine since ancient times. However, studies of S. buergeriana are still at a relatively early stage. Above all, there is still no established reference standard to control the quality of medicinal herbs and their products from S. buergeriana in Vietnam. Therefore, we present the isolation of trans-cinnamic acid from the root of S. buergeriana which was subsequently established as a chemical reference standard in this study. The isolation was conducted by recrystallization and CC on silica gel using different eluents. The purity and quality of the isolated compound were evaluated by spectroscopic methods and high-performance liquid chromatography (HPLC). Furthermore, the compound was evaluated for homogeneity and assigned value to establish reference standards according to General requirements for the competence of reference material producer’s guideline (TCVN ISO 17034:2017), Appendix 3 of World Health Organization (WHO technical report No. 943:2006) and Statistical methods for use in proficiency testing by interlaboratory comparison (ISO 13528:2015). The research successfully demonstrated the isolation and establishment of trans-cinnamic acid as a chemical reference standard for S. buergeriana which was herein reported for the first time.
Luu NNK, Tran NM, Tran PT, Trinh DH. Isolation and establishment of trans-cinnamic acid as a reference standard from Radix Scrophularia buergeriana Miq. J Appl Pharm Sci. 2024. Online First. http://doi.org/10.7324/JAPS.2024.186113
1. Do TL. Medicinal plants and herbs of Vietnam. Hanoi, Vietnam: Medical Publisher; 2004. p 820−1.
2. Park SU, Chae YA, Facchini RJ. Genetic transformation of the figwort, Scrophularia buergeriana Miq., an oriental medicinal plant. Plant Cell Rep. 2003;21:1194−8. doi: https://doi.org/10.1007/s00299-003-0639-0
3. Pham HH. An illustrated floral of Vietnam. Band 2. Ho Chi Minh City, Vietnam: Youth Publishing House; 2003. p 927−8.
4. Lee HJ, Lee DY, Kim HL, Yang SH. Scrophularia buergeriana extract improves memory impairment via inhibition of the apoptosis pathway in the mouse hippocampus. Appl Sci. 2020;10(22):7987−8000. doi: https://doi.org/10.3390/app10227987
5. Vietnam Standard and Quality. ISO/IEC 17034: General requirements for the competence of reference material producers [Internet]. 2017. Available from: https://caselaw.vn/van-ban-phap-luat/345574-tieu-chuan-quoc-gia-tcvn-iso-17034-2017-iso-17034-2016-ve-yeu-cau-chung-ve-nang-luc-cua-nha-san-xuat-mau-chuan-nam-2017?vanbangoc=true
6. International Organization for Standardization. ISO13528: Statistical methods for use in proficiency testing by interlaboratory comparison [Internet]. 2015. Available from: https://www.iso.org/standard/56125.html
7. Vietnamese pharmacopoeia V. Band 2. Hanoi, Vietnam: Medical Publishing House; 2018. p 1199−201.
8. Pham TV, Hoang HNT, Nguyen HT, Nguyen HM, Huynh CT, Vu TY, et al. Anti-inflammatory and antimicrobial activities of compounds isolated from Distichochlamys benenica. Biomed Res Int. 2021;2021:1−10. doi: https://doi.org/10.1155/2021/6624347
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