The oils obtained by hydrodistillation from fresh and dried aerial parts of Nepeta pungens Benth. at the flowering stage were analyzed by GC and GC-MS to investigate the variations of oil yields, oil components along with their percentages in fresh and dry stages. Fortyninecompounds (97.2%) were determined. The major compounds were geranyl acetate ( 17.0 %), limonene (12.0 %), eucalyptol (5.8 %), (bornylacetate (5.3 %), citronellal (4.9%), spathulanol (4.2%), sabinene (3.9%), β-ocimene (3.9), β-sesquiphellandrene (2.8%), nerylacetate (2.5%), α- humulene (2.4%), α-pinene(2.3%), humuleneoxide (2.2%), norsolanadione (2.1%) and terpinen-4- ol (2.0%). The yield of the oil was 1.1 (v/w) %. The essential oil showed antibacterial activity for Staphylococcus aureus.