The beneficial effect of the ethanolic extract Etlingera elatior fruit on IL-1ß and caspase-3 levels in sepsis model mice

Evi Nurhayatun Bambang Purwanto Soetrisno Soetrisno Dono Indarto Eti Poncorini Tatar Sumandjar Febriola Hotmaida Sagala Dalilah Salsabila Salma   

Open Access   

Published:  Feb 08, 2023

DOI: 10.7324/JAPS.2023.90410
Abstract

Etlingera elatior contains various active compounds with anti-inflammatory, antioxidant, and antibacterial effects. In sepsis, endothelial dysfunction can occur due to oxidative stress and hyperinflammation. This study evaluated the effect of E. elatior (EE) ethanolic extract in reducing IL-1ß and caspase-3 levels in sepsis model mice. Thirty-two Mus musculus mice (n = 32) were randomly distributed into four groups (n = 8). Induction of sepsis was performed through intraperitoneal injection of lipopolysaccharide (LPS) 0.3 mg/kgBW. Ethanol extract of EE 4.2 mg/20 g, p.o. was given 5 days before (EE-1), 5 days after (EE-2), and simultaneously during LPS induction (EE-3). ELISA was used to measure IL-1ß and caspase-3 levels. The ANOVA was performed to test the differences of each group. Mean levels of IL-1ß (p < 0.05) and caspase-3 (p < 0.05) differed in the control group with each intervention group. The EE significantly reduced levels of IL-1ß and caspase-3 (p < 0.05), with the best results being before LPS induction. This study provides that EE may have a role in averting endothelial dysfunction in sepsis by lowering levels of IL-1ß and caspase-3.


Keyword:     Etlingera elatior endothelial dysfunction interleukin-1ß caspase-3 sepsis


Citation:

Nurhayatun E, Purwanto B, Soetrisno S, Indarto D, Pon­corini E, Sumandjar T, Sagala FH, Salma DS. The benefi­cial effect of the ethanolic extract Etlingera elatior on IL-1ß and caspase-3 levels in sepsis model rats. J Appl Pharm Sci, 2023. https://doi.org/10.7324/JAPS.2023.90410

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Anzian AB, Sukor R, Saari N, Sapawi CW, Hussin AS. Chemical composition and antioxidant activity of Torch Ginger (Etlingera elatior) flower extract. Food Appl Biosci J, 2017; 5:32-49.

Aziz M, Varghese AJ, Health N, Yang W, Matsuda A. Current trends in inflammatory and immunomodulatory mediators in sepsis. J Leukoc Biol, 2012; https://doi.org/10.1189/jlb.0912437. https://doi.org/10.1189/jlb.0912437

Boisramé-Helms J, Kremer H, Schini-Kerth V, Meziani F. Endothelial dysfunction in sepsis. Curr Vasc Pharmacol, 2013; 11:150-60. https://doi.org/10.2174/1570161111311020005

Cecconi M, Evans L, Levy M, Rhodes A. Sepsis and septic shock. Lancet, 2018; 392:75-87; https://doi.org/10.1016/S0140-6736(18)30696-2. https://doi.org/10.1016/S0140-6736(18)30696-2

Chang JC. Sepsis and septic shock: endothelial molecular pathogenesis associated with vascular microthrombotic disease. Thromb J, 2019; 17:1-19; https://doi.org/10.1186/s12959-019-0198-4. https://doi.org/10.1186/s12959-019-0198-4

Dewi AR, Nur'Aini I, Bahri IS, Afifah HN, Fattah A, Tunjung WAS. Antihyperuricemic activity of ginger flower (Etlingera elatior Jack.) extract in beef broth-induced hyperuricemic rats (Rattus norvegicus). AIP Conf Proc, 2016; 1755; https://doi.org/10.1063/1.4958573. https://doi.org/10.1063/1.4958573

Dolmatova EV, Wang K, Mandavilli R, Griendling KK. The effects of sepsis on endothelium and clinical implications. Cardiovasc Res, 2021; 117:60-73; https://doi.org/10.1093/cvr/cvaa070. https://doi.org/10.1093/cvr/cvaa070

Dugar S, Choudhary C, Duggal A. Sepsis and septic shock: guideline-based management. Cleve Clin J Med, 2020; 87:53-64; https:// doi.org/10.3949/ccjm.87a.18143. https://doi.org/10.3949/ccjm.87a.18143

Ernilasari, Walil K, Fitmawati, Roslim DI, Zumaidar, Saudah, Rayhannisa. Antibacterial activity of leaves, flowers, and fruits extract of Etlingera elatior from Nagan Raya district, Indonesia against Escherichia coli and Staphylococcus aureus. Biodiversitas, 2021; 22:4457-64; https:// doi.org/10.13057/biodiv/d221039. https://doi.org/10.13057/biodiv/d221039

Farida S, Maruzy A. Kecombrang (Etlingera elatior): sebuah tinjauan penggunaan secara tradisional, fitokimia dan aktivitas farmakologinya. J Tumbuh Obat Indones, 2016; 9:19-28; https://doi. org/10.22435/toi.v9i1.6389.19-28. https://doi.org/10.22435/toi.v9i1.6389.19-28

Ghasemzadeh A, Jaafar HZE, Rahmat A, Ashkani S. Secondary metabolites constituents and antioxidant, anticancer and antibacterial activities of Etlingera elatior (Jack) R.M.Sm grown in different locations of Malaysia. BMC Complement Altern Med, 2015; 15:1-10; https://doi. org/10.1186/s12906-015-0838-6. https://doi.org/10.1186/s12906-015-0838-6

Gyawali B, Ramakrishna K, Dhamoon AS. Sepsis: the evolution in definition, pathophysiology, and management. SAGE Open Med, 2019; 7:205031211983504; https://doi.org/10.1177/2050312119835043. https://doi.org/10.1177/2050312119835043

Heerebeek L, Meischl C, Stooker W, Meijer CJLM, Niessen HWM, Roos D. NADPH oxidase(s): new source(s) of reactive oxygen species in the vascular system ? J Clin Pathol, 2002; 55(8):561-8. https://doi.org/10.1136/jcp.55.8.561

Hotchkiss R, Moldawer LL, Opal SM, Reinhart K, Tumbull IR, Vincent JL. Sepsis and septic shock. Nat Rev Dis Prim, 2017; 2:1-46; https://doi.org/10.1038/nrdp.2016.45.Sepsis. https://doi.org/10.1038/nrdp.2016.45

Hu R, Wu S, Li B, Tan J, Yan J, Wang Y, Tang Z, Liu M, Fu C, Zhang H, He J. Dietary ferulic acid and vanillic acid on inflammation, gut barrier function and growth performance in lipopolysaccharide-challenged piglets. Anim Nutr, 2022; 8:144-52; https://doi.org/https://doi. org/10.1016/j.aninu.2021.06.009. https://doi.org/10.1016/j.aninu.2021.06.009

Ince C, Mayeux PR, Nguyen T, Gomez H, Kellum JA, Ospina- Tascón GA, Hernandez G, Murray P, De Backer D. The endothelium in sepsis. Shock, 2016; 45:259-70; https://doi.org/10.1097/SHK.0000000000000473 https://doi.org/10.1097/SHK.0000000000000473

Isyanti M, Andarwulan N, Faridah DN. Karakteristik Fisik dan Fitokimia Buah Kecombrang (Etlingera elatior). J Agro-Based Ind, 2019; 36:96-105. https://doi.org/10.32765/wartaihp.v36i2.5267

Jacobi J. The pathophysiology of sepsis-2021 update: Part 1, immunology and coagulopathy leading to endothelial injury. Am J Health Syst Pharm. 2022 Feb 18;79(5):329-337. doi: 10.1093/ajhp/zxab380. PMID: 34605875; PMCID: PMC8500113. https://doi.org/10.1093/ajhp/zxab380

Juwita T, Pakpahan WHP, Puspitasari IM, Saptarini NM, Levita J. Anti-inflammatory activity of Etlingera elatior (Jack) r.m. smith flower on gastric ulceration-induced wistar rats. Pakistan J Biol Sci, 2020; 23:1193-200; https://doi.org/10.3923/pjbs.2020.1193.1200. https://doi.org/10.3923/pjbs.2020.1193.1200

Juwita T, Puspitasari IM, Levita J. Torch ginger (Etlingera elatior): a review on its botanical aspects, phytoconstituents and pharmacological activities. Pak J Biol Sci, 2018; 21:151-65; https://doi. org/10.3923/PJBS.2018.151.165. https://doi.org/10.3923/pjbs.2018.151.165

Leorita M, Mardikasari SA, Wahyuni, Malaka MH, Sartinah A, Sahidin. Aktivitas Antioksidan dan Toksisitas Akut Ekstrak Etanol Buah, Daun, Batang dan Rimpang Tanaman Wualae (Etlingera elatior (Jack) R.M. Smith). J Farm Sains Dan Kesehat, 2018; 4:36-9. https://doi.org/10.33772/pharmauho.v4i2.6263

Lin J, Li H, Wen Y, Zhang M. Adjuvant administration of vitamin C improves mortality of patients with sepsis and septic shock: a systems review and meta-analysis. Open J Intern Med, 2018; 08:146-59; https://doi. org/10.4236/ojim.2018.82015. https://doi.org/10.4236/ojim.2018.82015

Lorente L, Martín MM, Pérez-Cejas A, González-Rivero AF, López RO, Ferreres J, Solé-Violán J, Labarta L, Díaz C, Palmero S, Jiménez A. Sustained high serum caspase-3 concentrations and mortality in septic patients. Eur J Clin Microbiol Infect Dis, 2018; 37:281-8; https://doi. org/10.1007/s10096-017-3129-y. https://doi.org/10.1007/s10096-017-3129-y

Luan YY, Yao YM, Xiao XZ, Sheng ZY. Insights into the apoptotic death of immune cells in sepsis. J Interf Cytokine Res, 2015; 35:17-22; https://doi.org/10.1089/jir.2014.0069. https://doi.org/10.1089/jir.2014.0069

Namas R, Zamora R, Namas R, An G, Doyle J, Dick TE, Jacono FJ, Androulakis IP, Nieman GF, Chang S, Billiar TR, Kellum JA, Angus DC, Vodovotz Y. Sepsis: something old, something new, and a systems view. J Crit Care, 2012; 27:314. e1-314e.11; https://doi.org/10.1016/j. jcrc.2011.05.025. https://doi.org/10.1016/j.jcrc.2011.05.025

Nasronudin. Penyakit infeksi di Indonesia Solusi Kini dan Mendatang. 2nd edition, Percetakan UNAIR, Surabaya, Indonesia, 2011.

Prescott HC, Angus DC. Enhancing recovery from sepsis: a review. JAMA J Am Med Assoc, 2018; 319:62-75; https://doi.org/10.1001/ jama.2017.17687. https://doi.org/10.1001/jama.2017.17687

Sahidin S, Salsabila S, Wahyuni W, Adryan F, Imran I. Potensi Antibakteri Ekstrak Metanol dan Senyawa Aromatik dari Buah Wualae (Etlingera elatior). J Kim Val, 2019; 5:1-7; https://doi.org/10.15408/jkv. v5i1.8658. https://doi.org/10.15408/jkv.v5i1.8658

Shioiri T, Muroi M, Hatao F, Nishida M, Ogawa T, Mimura Y, Seto Y, Kaminishi M, Tanamoto KI. Caspase-3 is activated and rapidly released from human umbilical vein endothelial cells in response to lipopolysaccharide. Biochim Biophys Acta Mol Basis Dis, 2009; 1792:1011-8; https://doi.org/10.1016/j.bbadis.2009.06.006. https://doi.org/10.1016/j.bbadis.2009.06.006

Srey C, Sontimuang C, Thengyai S, Ovatlarnporn C, Puttarak P. Anti α-glucosidase, anti α-amylase, anti-oxidation, and anti-inflammation activities of Etlingera elatior rhizome. J Chem Pharm Res, 2014; 6:885-91. Available via WwwJocprCom

Tundis R, Loizzo MR, Menichini F. Natural products as alpha-amylase and alpha-glucosidase inhibitors and their hypoglycaemic potential in the treatment of diabetes: an update. Mini-Rev Med Chem, 2010; 10:315- 31; https://doi.org/10.2174/138955710791331007. https://doi.org/10.2174/138955710791331007

Wahyuni W, Malik F, Ningsih A, Zubaydah WOS, Sahidin S. Antimicrobial activities of ethanol extract of Wualae (Etlingera elatior (JACK) R.M. Smith). J Pharm Med Sci, 2018; 3:14-8.

Wu F, Schuster DP, Tyml K, Wilson JX. Ascorbate inhibits NADPH oxidase subunit p47phox expression in microvascular endothelial cells. Free Radic Biol Med, 2007; 42:124-31; https://doi.org/10.1016/j. freeradbiomed.2006.10.033. https://doi.org/10.1016/j.freeradbiomed.2006.10.033

Yani Y, Tri Arianto A, Sudjito M. Pengaruh Pemberian Propofol Intravena terhadap Ekspresi Kaspase 3 Hipokampus pada Mencit Balb/C dengan Cedera Kepala. J Neuroanestesi Indones, 2013; 2:81-8; https://doi. org/10.24244/jni.vol2i2.160. https://doi.org/10.24244/jni.vol2i2.160

Yousefian M, Shakour N, Hosseinzadeh H, Hayes AW, Hadizadeh F, Karimi G. The natural phenolic compounds as modulators of NADPH oxidases in hypertension. Phytomedicine, 2019; 55:200-13; https://doi.org/10.1016/j.phymed.2018.08.002. https://doi.org/10.1016/j.phymed.2018.08.002

Yu D, Unger D, Unge C, Parke Å, Sundén-Cullberg J, Strålin K, Özenci V. Correlation of clinical sepsis definitions with microbiological characteristics in patients admitted through a sepsis alert system; a prospective cohort study. Ann Clin Microbiol Antimicrob, 2022; 21:1-9; https://doi.org/10.1186/s12941-022-00498-3. https://doi.org/10.1186/s12941-022-00498-3

Article Metrics

0 Absract views 2 PDF Downloads 2 Total views

   Abstract      Pdf Download

Related Search

By author names

Citiaion Alert By Google Scholar

Name Required
Email Required Invalid Email Address

Comment required