The beneficial effect of the ethanolic extract Etlingera elatior fruit on IL-1ß and caspase-3 levels in sepsis model mice

Evi Nurhayatun; Bambang Purwanto; Soetrisno Soetrisno; Dono Indarto; Eti Poncorini; Tatar Sumandjar; Febriola Hotmaida Sagala; Dalilah Salsabila Salma

doi:10.7324/JAPS.2023.90410

Abstract

Etlingera elatior contains various active compounds with anti-inflammatory, antioxidant, and antibacterial effects. In sepsis, endothelial dysfunction can occur due to oxidative stress and hyperinflammation. This study evaluated the effect of E. elatior (EE) ethanolic extract in reducing IL-1ß and caspase-3 levels in sepsis model mice. Thirty-two Mus musculus mice (n = 32) were randomly distributed into four groups (n = 8). Induction of sepsis was performed through intraperitoneal injection of lipopolysaccharide (LPS) 0.3 mg/kgBW. Ethanol extract of EE 4.2 mg/20 g, p.o. was given 5 days before (EE-1), 5 days after (EE-2), and simultaneously during LPS induction (EE-3). ELISA was used to measure IL-1ß and caspase-3 levels. The ANOVA was performed to test the differences of each group. Mean levels of IL-1ß (p < 0.05) and caspase-3 (p < 0.05) differed in the control group with each intervention group. The EE significantly reduced levels of IL-1ß and caspase-3 (p < 0.05), with the best results being before LPS induction. This study provides that EE may have a role in averting endothelial dysfunction in sepsis by lowering levels of IL-1ß and caspase-3.

Keyword: Etlingera elatior endothelial dysfunction interleukin-1ß caspase-3 sepsis

Citation:

Nurhayatun E, Purwanto B, Soetrisno S, Indarto D, Poncorini E, Sumandjar T, Sagala FH, Salma DS. The beneficial effect of the ethanolic extract Etlingera elatior on IL-1ß and caspase-3 levels in sepsis model rats. J Appl Pharm Sci, 2023. https://doi.org/10.7324/JAPS.2023.90410

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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