Antimicrobial activity of chlorhexidine on Candida albicans and Streptococcus mutans isolated in children with early childhood caries – An in vitro study

Nipun Jain Balagopal R Varma Suresh J Kumar Parvathy Kumaran Arun Mamachan Xavier   

Open Access   

Published:  Nov 15, 2022

DOI: 10.7324/JAPS.2023.130213

Streptococcus mutans and Candida albicans have a unique microbial feature that plays an essential role in early childhood caries (ECC). This study aimed to isolate C. albicans and S. mutans in children with ECC and observe the antimicrobial effect of 0.2% chlorhexidine (CHX). Children in the age group of 3–6 years were selected. The sample was collected using a sterile cotton swab from the tooth surface, streaked on sabouraud dextrose agar (HIMedia) plates, and incubated at 37°C for 24 hours. Disk diffusion and agar well diffusion methods were used to detect the susceptibility of C. albicans and S. mutans to 0.2% CHX. The mean zone of inhibition of CHX for C. albicans was 12.4 ± 0.59 mm, and S. mutans showed a zone of inhibition of 20.85 ± 1.18 mm. CHX was effective against both C. albicans and S. mutans, showing more antibacterial activity than antifungal activity.

Keyword:     Chlorhexidine gluconate Candida albicans Streptococcus mutans early childhood caries antimicrobial activity


Jain N, Varma RB, Kumar JS, Kumaran P, Xavier AM. Antimicrobial activity of chlorhexidine on Candida albicans and Streptococcus mutans isolated in children with early childhood caries – An in vitro study. J Appl Pharm Sci, 2022.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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