Green synthesis of silver nanoparticles (AgNPs) using microbial enzymes has gained great attention owing to its advantages, such as cost-effectiveness, biocompatibility, eco-friendliness, and simplicity, over conventional physical and chemical methods. In our study, the laccase enzyme of an unconventional source like the endophytic strain Alternaria arborescens MK629314 was optimized and applied for AgNP biogenesis. Alternaria arborescens laccase enzyme production was first improved using a three-factor, five-level central composite design of 20 trails; the low-cost medium of rice bran increased the laccase production to 6.5-fold. Second, the semipurified laccase enzyme was applied as a reducing and capping agent for the synthesis of AgNPs, and all the characteristics of the formed nanoparticles were studied. The applied techniques were transmission electron microscopy, UV-vis spectroscopy, Fourier transform infrared (FTIR), and dynamic light scattering (DLS). The results of all the analyses emphasized the formation of AgNPs. AgNPs biosynthesized from 25% and 50% semipurified enzyme fractions had size ranges of 6.51 ± 5.12 and 2.51 ± 3.45 nm and showed UV-characteristic peaks at wavelengths of 416 and 404 nm, respectively. DLS analysis presented good peaks with particle size means of 96.20 and 82.30 nm and zeta potentials of −58.3 and −98.5 mv. FTIR analysis showed the appearance of distinctive bands at 3,849.22, 3,440.39, 1,637.27, 1,432.85, 1,159.01, 1,031.73, 597.825, and 520.686 cm−1. The well diffusion method showed a good inhibition zone diameter of antibacterial efficiency against Escherichia coli and Staphylococcus aureus (15–17 mm) and antifungal activity against filamentous fungi Aspergillus niger and Fusarium solani (11–13 mm). The achieved results add to the growing relevance of A. arborescens as a potential applied endophytic fungus used for diverse biomedical applications.
Alharbi R M, Alshammari SO, Abd El Aty AA. Statically improved fungal laccase-mediated biogenesis of silver nanoparticles with antimicrobial applications. J Appl Pharm Sci, 2022. https://doi.org/10.7324/JAPS.2023.130105
Abd El Aty AA, Ammar HA. Potential characterization and antimicrobial applications of newly bio-synthesized silver and copper nanoparticles using the novel marine-derived fungus Alternaria tenuissima KM651985. Res J BioTechnol, 2016; 11(8):71-82.
Abd El Aty AA, El-Shamy AR, Atalla SMM, El-Diwany AI, Hamed ER. Screening of fungal isolates for laccase enzyme production from marine sources. Res J Pharm Biol Chem Sci, 2015; 6:221-8.
Abd El Aty AA, Hamed ER, El-Beih AA, El-Diwany AI. Induction and enhancement of the novel marine-derived Alternaria tenuissima KM651985 laccase enzyme using response surface methodology: application to azo and triphenylmethane dyes decolorization. J Appl Pharm Sci, 2016; 6(4):6-14.
Abd El Aty AA, Mohamed AA, Zohair MM, Soliman AAF. Statistically controlled biogenesis of silver nano-size by Penicillium chrysogenum MF318506 for biomedical application. Biocatal Agric Biotechnol, 2020; 25:101592; doi:10.1016/j.bcab.2020.101592https://doi.org/10.1016/j.bcab.2020.101592
Abd El Aty AA, Mostafa FA. Effect of various media and supplements on laccase activity and its application in dyes decolorization. Malays J Microbiol, 2013; 9(2):166-75.
Abd El Aty AA, Mostafa FA, Hassan ME, Hamed ER, Esawy MA. Covalent immobilization of Alternaria tenuissima KM651985 laccase and some applied aspects. Biocatal Agric Biotechnol, 2017; 9:74-81.https://doi.org/10.1016/j.bcab.2016.12.001
Abd El Aty AA, Shehata AN, Shaheen TI. Production and sequential optimization of Bacillus subtilis MF467279 pullulanase by statistical experimental designs and evaluation of its desizing efficiency. Biocatal Agric Biotechnol, 2018; 14:375-85.https://doi.org/10.1016/j.bcab.2018.04.004
Abd El Aty AA, Zohair MM. Green-synthesis and optimization of an eco-friendly nanobiofungicide from Bacillus amyloliquefaciens MH046937 with antimicrobial potential against phytopathogens. Environ Nanotechnol Monit Manage, 2020; 14:100309; doi:10.1016/j.enmm.2020.100309https://doi.org/10.1016/j.enmm.2020.100309
Adelere IA, Lateef A. A novel approach to the green synthesis of metallic nanoparticles: the use of agro-wastes, enzymes, and pigments. Nanotechnol Rev, 2016; 5(6):567-87.https://doi.org/10.1515/ntrev-2016-0024
Alshammari SO, Abd El Aty AA. Statically controlled mycogenic-synthesis of novel biologically active silver-nanoparticles using Hafr Al-Batin desert truffles and its antimicrobial efficacy against pathogens. Saudi J Biol Sci, 2022; 29:103334; doi:10.1016/j.sjbs.2022.103334https://doi.org/10.1016/j.sjbs.2022.103334
Ammar HA, Abd El Aty AA, El Awdan SA. Extracellular myco synthesis of nano silver using the fermentable yeasts Pichia kudriavzevii HA NY2 and Saccharomyces uvarum HA NY3, and their effective biomedical applications. Bioprocess Biosyst Eng, 2021; 44:841- 54; doi:10.1007/s00449-020-02494-3https://doi.org/10.1007/s00449-020-02494-3
Bassanini I, Ferrandi EE, Riva S, Monti D. Biocatalysis with laccases: an updated overview. Catalysts, 2021; 11(26):1-30; doi:10.3390/ catal11010026https://doi.org/10.3390/catal11010026
Behera A, Pradhan SP, Ahmed FK, Abd-Elsalam KA. Chapter 28-enzymatic synthesis of silver nanoparticles: mechanisms, and applications. Green synthesis of silver nanomaterials nanobiotechnology for plant protection, pp 699-756, 2022; doi:10.1016/B978-0-12-824508-8.00030-7https://doi.org/10.1016/B978-0-12-824508-8.00030-7
Bhat R, Deshpande R, Ganachari SV, Huh DS, Venkataraman A. Photo-irradiated biosynthesis of silver nanoparticles using edible
mushroom Pleurotus florida and their antibacterial activity. Bioinorg Chem Appl, 2011; 2011:650979.
Chaurasia PK, Bharati SL, Yadava S. Nano-reduction of gold and silver ions: a perspective on the fate of microbial laccases as potential biocatalysts in the synthesis of metals (gold and silver) nano-particles. Curr Res Microbial Sci, 2022; 3:100098; doi:10.1016/j.crmicr.2021.100098https://doi.org/10.1016/j.crmicr.2021.100098
Chawachart N, Khanongnuch C, Watanabe T, Lumyong S. Rice bran as an efficient substrate for laccase production from thermotolerant basidiomycete Coriolus versicolor strain RC3. Fungal Divers, 2004; 15:23-32.
Cottenie A, Verloo L, Kiens L, Velghe G, Camerlynch R. Chemical analysis of plants and soils. Laboratory of Analytical and Agrochemistry, Ghent, Belgium, 1982.
Devasia S, Nair AJ. Screening of potent laccase producing organisms based on the oxidation pattern of different phenolic substrates. Int J Curr Microbiol App Sci, 2016; 5(5):127-37.https://doi.org/10.20546/ijcmas.2016.505.014
Eid AM, Fouda A, Niedba?a G, Hassan SE-D, Salem SS, Abdo AM, Hetta HF, Shaheen TI. Endophytic Streptomyces laurentii mediated green synthesis of Ag-NPs with antibacterial and anticancer properties for developing functional textile fabric properties. Antibiotics, 2020; 9:641.https://doi.org/10.3390/antibiotics9100641
Elegbede JA, Lateef A, Azeez MA, Asafa TB, Yekeen TA, Oladipo IC, Adebayo EA, Beukes LS, Gueguim-Kana EB. Fungal xylanases-mediated synthesis of silver nanoparticles for catalytic and biomedical applications. IET Nanobiotechnol, 2018; 12(6):857-63.https://doi.org/10.1049/iet-nbt.2017.0299
Eltarahony M, Zaki S, El-Haleem DA. Concurrent synthesis of zero-and one-dimensional, spherical, rod-, needle-, and wire-shaped CuO nanoparticles by Proteus mirabilis 10B. J Nanomater, 2018; 2018:1849616; doi:10.1155/2018/1849616https://doi.org/10.1155/2018/1849616
Eltarahony M, Zaki S, Abd-El-Haleem D. Aerobic and anaerobic removal of lead and mercury via calcium carbonate precipitation mediated by statistically optimized nitrate reductases. Sci Rep, 2020; 10:4029; doi:10.1038/s41598-020-60951-1https://doi.org/10.1038/s41598-020-60951-1
Eltarahony M, Ibrahim A, El-shall H, Ibrahim E, Althobaiti F, Fayad E. Antibacterial, antifungal and antibiofilm activities of silver nanoparticles supported by crude bioactive metabolites of bionanofactories isolated from lake mariout. Molecules, 2021; 26:3027; doi:10.3390/ molecules26103027https://doi.org/10.3390/molecules26103027
Elyamny S, Eltarahony M, Abu Serie M, Nabil MM, Kashyout AEHB. One pot fabrication of Ag @ Ag2O core-shell nanostructures for biosafe antimicrobial and antibioflm applications. Sci Rep, 2021; 11:22543.https://doi.org/10.1038/s41598-021-01687-4
Faramarzi MA, Forootanfar H. Biosynthesis and characterization of gold nanoparticles produced by laccase from Paraconiothyrium variabile. Colloids Surf B Biointerf, 2011; 87:23-7.https://doi.org/10.1016/j.colsurfb.2011.04.022
Gopinath V, Velusamy P. Extracellular biosynthesis of silver nanoparticles using Bacillus sp. GP-23 and evaluation of their antifungal activity towards Fusarium oxysporum. Spectrochim Acta A Mol Biomol Spectrosc, 2013; 106:170-4.https://doi.org/10.1016/j.saa.2012.12.087
Ibrahim A, El-Fakharany EM, Abu-Serie MM, ElKady MF, Eltarahony M. Methyl orange biodegradation by immobilized consortium microspheres: experimental design approach, toxicity study and bioaugmentation potential. Biology, 2022; 11:76; doi:10.3390/ biology11010076https://doi.org/10.3390/biology11010076
Kumar SA, Abyaneh MK, Gosavi SW, Kulkarni SK, Ahmad A, Khan MI. Sulfite reductase-mediated synthesis of gold nanoparticles capped with phytochelatin. Biotechnol Appl Biochem, 2007; 47:191-5.https://doi.org/10.1042/BA20060205
Lateef A, Adeeyo AO. Green synthesis and antibacterial activities of silver nanoparticles using extracellular laccase of Lentinus edodes. Not Sci Biol, 2015; 7:405-11; doi:10.15835/nsb.7.4.9643https://doi.org/10.15835/nsb.7.4.9643
Lateef A, Akande MA, Ojo SA, Folarin BI, Gueguim-kana EB, Beukes LS. Paper wasp nest-mediated biosynthesis of silver nanoparticles for antimicrobial, catalytic, anticoagulant, and thrombolytic applications. 3 Biotech, 2016; 6:140.https://doi.org/10.1007/s13205-016-0459-x
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J Biol Chem, 1951; 193:265-75.https://doi.org/10.1016/S0021-9258(19)52451-6
Mayolo-Deloisa K, Gonz ?alez-Gonz ?alez M, Rito-Palomares M. Laccases in food industry: bioprocessing, potential industrial and biotechnological applications. Front Bioeng Biotechnol, 2020; 8:222; doi:10.3389/fbioe.2020.00222https://doi.org/10.3389/fbioe.2020.00222
Mostafa FA, Abd El Aty AA. Thermodynamics enhancement of Alternaria tenuissima KM651985 laccase by covalent coupling to polysaccharides and its applications. Int J Biol Macromol, 2018; 120:222-9.https://doi.org/10.1016/j.ijbiomac.2018.08.081
Mulvaney P. Surface plasmon spectroscopy of nanosized metal particles. Langmuir, 1996; 12:788-800.https://doi.org/10.1021/la9502711
Munoz C, Guillen F, Martinez AT, Martinez MJ. Induction and characterization of laccase in the lininolytic fungus Pleurotus eryngii. Curr Microbiol, 1997; 34:1-5.https://doi.org/10.1007/s002849900134
Muthukumarasamy NP, Jackson B, Raj AJ, Sevanan M. Production of extracellular laccase from Bacillus subtilis MTCC 2414 using agroresidues as a potential substrate. Biochem Res Int, 2015; 2015:765190; doi:10.1155/2015/765190https://doi.org/10.1155/2015/765190
Ovais M, Khalil AT, Ayaz M, Ahmad I, Nethi SK, Mukherjee S. Biosynthesis of metal nanoparticles via microbial enzymes: a mechanistic approach. Int J Mol Sci, 2018; 19:4100; doi:10.3390/ijms1912410.https://doi.org/10.3390/ijms19124100
Patel H, Gupte A. Optimization of different culture conditions for enhanced laccase production and its purification from Tricholoma giganteum AGHP. Bioresour Bioprocess, 2016; 3:11; doi:10.1186/s40643- 016-0088-6https://doi.org/10.1186/s40643-016-0088-6
Rai T, Panda D. An extracellular enzyme synthesizes narrowsized silver nanoparticles in both water and methanol. Chem Phys Lett, 2015; 623:108-12.https://doi.org/10.1016/j.cplett.2015.02.003
Raju D, Paneliya N, Mehta UJ. Extracellular synthesis of silver nanoparticles using living peanut seedling. Appl Nanosci, 2014; 4:875-9; doi:10.1007/s13204-013-0269-yhttps://doi.org/10.1007/s13204-013-0269-y
Rangnekar A, Sarma TK, Singh AK, Deka J, Ramesh A, Chattopadhyay A. Retention of enzymatic activity of α-amylase in the reductive synthesis of gold nanoparticles. Langmuir, 2007; 23:5700-6.https://doi.org/10.1021/la062749e
Sanket S, Das SK. Role of enzymes in synthesis of nanoparticles. In: Thatoi H, Mohapatra S, Das SK (eds.). Bioprospecting of enzymes in industry, healthcare and sustainable environment, Springer, The Gateway, Singapore, pp 139-53, 2021; doi:10.1007/978-981-33-4195-1_7https://doi.org/10.1007/978-981-33-4195-1_7
Sanghi R, Verma P, Puri S. Enzymatic formation of gold nanoparticles using Phanerochaete chrysosporium. Adv Chem Eng Sci, 2011; 1:154-62.https://doi.org/10.4236/aces.2011.13023
Shaheen TI, Abd El Aty AA. In-situ green myco-synthesis of silver nanoparticles onto cotton fabrics for broad spectrum antimicrobial activity. Int J Biol Macromol, 2018; 118:2121-30.https://doi.org/10.1016/j.ijbiomac.2018.07.062
Shaligram NS, Singh SK, Singhal RS, Szakacs G, Pandey A. Effect of precultural and nutritional parameters on compactin production by solid state fermentation. J Microbiol Biotechnol, 2009; 19:690-7.https://doi.org/10.4014/jmb.0808.466
Shehata AN, Abd El Aty AA. Optimization of process parameters by statistical experimental designs for the production of naringinase enzyme by marine fungi. Int J Chem Eng, 2014; 2014:273523; doi:10.1155/2014/273523https://doi.org/10.1155/2014/273523
Sousa AC, Martins LO, Robalo MP. Laccases: versatile biocatalysts for the synthesis of heterocyclic cores. Molecules, 2021; 26(12):3719; doi:10.3390/molecules26123719https://doi.org/10.3390/molecules26123719
Thirumurugan A, Tomy NA, Kumar HP, Prakash P. Biological synthesis of silver nanoparticles by Lantana camara leaf extracts. Int J Nanomater Bioresour, 2011; 1:22-4.
Tortella GR, Rubilar O, Gianfreda L, Valenzuela E, Diez MC. Enzymatic characterization of chilean native wood-rotting fungi for potential use in the bioremediation of polluted environments with chlorophenols. World J Microbiol Biotechnol, 2008; 12:2805-18.https://doi.org/10.1007/s11274-008-9810-7
0 Absract views 3 PDF Downloads 3 Total views