Neutralization of Naja naja and Daboia russelii snake venoms by aqueous plant extracts

Adwait M. Deshpande K. Venkata Sastry Satish B. Bhise   

Open Access   

Published:  Jun 23, 2022

Abstract

Traditional Indian literature enlists many methods and agents to treat snake envenomation, but most of these methods are not pharmacologically investigated. This study evaluates snake venom-antagonizing properties of the aqueous extracts of five plants, viz. Sapindus laurifolius, Spondias pinnata, Plumeria lutea, Woodfordia fruticosa, and Croton roxburghii, against the venoms of the spectacled cobra and Russell’s viper. Inhibitions of venom lethality, hemorrhage, coagulation, and enzymes were studied to determine the action of the plant extracts. Median lethal dose values of Naja naja and Daboia russelii venoms in mice were 0.625 and 4.0 mg/kg, respectively, when determined as per the modified guidelines by Organisation for Economic Co-operation and Development (OECD). Woodfordia fruticosa presented ~20- and ~5-fold neutralization capacities in vitro and in vivo, respectively. Plant extracts under investigation presented changes in hemorrhagic and coagulant activities in varying degrees. Spondias pinnata and W. fruticosa neutralized the activity of phospholipase A2 and acetylcholinesterase almost completely in vitro. Woodfordia fruticosa presented relatively fair results among the studied plants, and molecular investigations of its isolates can lead us to new molecules. Easy-to-administer drugs with better safety margins can be great lifesavers in remote places where the chances of a snake bite are higher and medical resources are sparse.


Keyword:     Naja naja venom Daboia russelii venom phospholipase A2 acetylcholinesterase plant-based anti-snake venom Woodfordia fruticosa.


Citation:

Deshpande AM, Sastry KV, Bhise SB. Neutralization of Naja naja and Daboia russelii snake venoms by aqueous plant extracts. J Appl Pharm Sci, 2022.Online First.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Reference

Alam MI, Gomes A. Snake venom neutralization by Indian medicinal plants (Vitex negundo and Emblica officinalis) root extracts. J Ethnopharmacol, 2003; 86(1):75-80. https://doi.org/10.1016/S0378-8741(03)00049-7

lmeida SM, Almeida MR. Dictionary of generic names of flowering plants and ferns in Maharashtra. Satyam Enterprises, Mumbai, India, 2005.

Arul B, Kothai R, Jacob P, Sangameswaran B, Sureshkumar K. Anti-inflammatory activity of Sapindus trifoliatus Linn. J Herb Pharmacother, 2004; 4(4):43-50. https://doi.org/10.1080/J157v04n04_04

Arya A, Abdullah MA, Haerian BS, Mohd MA. Screening for hypoglycemic activity on the leaf extracts of nine medicinal plants: in-vivo evaluation. E J Chem, 2012; 9(3):1196-205. https://doi.org/10.1155/2012/103760

Aslam N, Fatima S, Khalid S, Hussain S, Qayum M, Afzal K, Afzal K, Asad MHHB. Anti-5-Nucleotidases (5-ND) and acetylcholinesterase (AChE) activities of medicinal plants to combat Echis carinatus venom-induced toxicities. Biomed Res Int, 2021; 2021:10. https://doi.org/10.1155/2021/6631042

Chethankumar M, Srinivas L. New biological activity against phospholipase A2 by Turmerin, a protein from Curcuma longa L. J Biol Chem, 2008; 389:299-3303. https://doi.org/10.1515/BC.2008.024

Cousin X, Bon C. Acetylcholinesterase from snake venoms. C R Seances Soc Biol Fil, 1996; 191(3):381-400.

Deepa M, Gowda TV. Purification and characterization of a glycoprotein inhibitor of toxic phospholipase from Withania somnifera. Arch Biochem Biophys, 2002; 408:42-50. https://doi.org/10.1016/S0003-9861(02)00527-1

Ebrahimi Majdar R, Ghasemian A, Resalati H, Saraeian A, Crestini C, Lange H. Facile isolation of LCC-fraction from organosolv lignin by simple soxhlet extraction. Polymers, 2019; 11(2):225. https://doi.org/10.3390/polym11020225

Elnour EA, Abdmageed MAM, Shyoub ME, Mohammed RR. Evaluation of toxicity of some plants having traditional uses in Sudan on brine shrimp. Glob J Tradit Med Syst, 2013; 2(1):19-23.

Ghag-Sawant M, More TV, Samant LS, Chowdhary AS. Study of neutralization of enzymatic activity of Daboia russelii venom by various plant extracts and their combinations using in vitro methods. Int J Pharm Sci Res, 2016; 7(6):2531-6.

Gomes A, Das R, Sarkhel S, Mishra R, Mukherjee S, Bhattacharya S, Gomes A. Herbs and herbal constituents active against snake bite. Indian J Exp Biol, 2010; 48:865-78.

Gupta M, Mazumder UK, Vamsi MLM, Sivakumar T, Kandar CC. Anti-steroidogenic activity of the two Indian medicinal plants in mice. J Ethnopharmacol, 2004; 90(1):21-5. https://doi.org/10.1016/j.jep.2003.09.002

Houghton PJ, Osibogun IM. Flowering plants used against snakebite. J Ethnopharmacol, 1993; 39(1):1-29. https://doi.org/10.1016/0378-8741(93)90047-9

Inouye H, Okigawa M, Shimokawa N. Studies on monoterpene glucosides VIII artefacts formed during extraction of Asperuloside and Paederoside. Chem Pharm Bull, 1969; 17(9):1949-54. https://doi.org/10.1248/cpb.17.1949

Jayanthi GP, Gowda VT. Geographical variation in India in the composition and lethal potency of Russell's viper (Vipera russelli) venom. Toxicon, 1988; 26(3):257-64. https://doi.org/10.1016/0041-0101(88)90216-4

Khan H, Marya, Amin S, Kamal MA, Patel S. Flavonoids as acetylcholinesterase inhibitors: current therapeutic standing and future prospects. Biomed Pharmacother, 2018; 101:860-70. https://doi.org/10.1016/j.biopha.2018.03.007

Khyade MS, Takate YA, Divekar MV. Plants used as an antidote against snakebite in Akole Taluka of Ahmednagar District (MS), India. J Nat Remedies, 2011; 11(2):182-92.

Kini RM. Excitement ahead: structure, function and mechanism of snake venom phospholipase A2 enzymes. Toxicon, 2003; 42(8):827-40. https://doi.org/10.1016/j.toxicon.2003.11.002

The Lancet. Snake-bite envenoming: a priority neglected tropical disease. Lancet, 2017; 370(10089):P2. https://doi.org/10.1016/S0140-6736(17)31751-8

Lattig J, Bohl M, Fischer P, Tischer S, Tietbohl C, Menschikowski M, Gutzeit HO, Metz P, Pisabarro MT. Mechanism of inhibition of human secretory phospholipase A2 by flavonoids: rationale for lead design. J Comput Aided Mol Des, 2007; 21:473-83. https://doi.org/10.1007/s10822-007-9129-8

Martz W. Plants with a reputation against snakebite. Toxicon, 1992; 30(10):1131-42. https://doi.org/10.1016/0041-0101(92)90429-9

Mohapatra B, Warrell DA, Suraweera W, Bhatia P, Dhingra N, Jotkar RM, Rodriguez PS, Mishra K, Whitaker R, Jha P, Million Death Study Collaborators. Snakebite mortality in India: a nationally representative mortality survey. PLOS Negl Trop Dis, 2011; 5(4):e1018. https://doi.org/10.1371/journal.pntd.0001018

Mors WB, Célia do Nascimento M, Ruppelt Pereira BM, Pereira NA. Plant natural products active against snake bite-the molecular approach. Phytochemistry, 2000; 55(6):627-42. https://doi.org/10.1016/S0031-9422(00)00229-6

Mukherjee AK, Ghosal SK, Maity CR. Some biochemical properties of Russell's viper (Daboia russelli) venom from Eastern India: correlation with clinico-pathological manifestation in Russell's viper bite. Toxicon, 2000; 38(2):163-75. https://doi.org/10.1016/S0041-0101(99)00125-7

Ode OJ, Asuzu IU. The anti-snake venom activities of the methanolic extract of the bulb of Crinum jagus (Amaryllidaceae). Toxicon, 2006; 48(3):331-42. https://doi.org/10.1016/j.toxicon.2006.06.003

OECD. 423 Guidelines for the testing of chemicals: acute oral toxicity-acute toxic class method. Organisation for economic co-operation and development, 2001a. Available via https://ntp.niehs.nih.gov/iccvam/ suppdocs/feddocs/oecd/oecd_gl423.pdf (Accessed 22 May 2022).

OECD. 425 Guidelines for the testing of chemicals: acute oral toxicity-up-and-down procedure. Organisation for economic co-operation and development, 2001b.

Pade SD. Aryabhishak arthat hindusthancha vaidyaraj. Rajesh Prakashan, Pune, India, 1893a.

Pade SD. 1893b. Vanaushadhi Gunadarsha. Shri Gajanan Book Depot, Pune, India, 1893b.

Pade SD, Patil PB, Gadre DV, Padhye-Gurjar AB. Aushadhi Baad. Rajesh Prakashan, Pune, India, 2010.

Pal R, Mukherjee A, Saha A. Exploring post-coital anti-fertility activity with toxicological and hormonal profiling of Sapindus trifoliatus Linn. Int Res J Pharm Appl Sci, 2013; 3(5):53-60.

Panda BK, Patro VJ, Mishra US. Evaluation of diuretic activity of acetone and ethanol stem barks extracts of Spondias pinnata (Linn. F) Kurz in Rats. Int J Pharm Chem Sci, 2014; 3(2):589-93.

Pereanez JA, Nunez V, Patino AC, Londono M, Quintana JC. Inhibitory effects of plant phenolic compounds on enzymatic and cytotoxic activities induced by a snake venom phospholipase A2. Vitae, Revista De La Facultad De Química Farmacéutica, 2011; 18(3):295-304.

Punde DP. Management of snake-bite in rural Maharashtra: a 10-year experience. Natl Med J India, 2005; 18(2):71.

Roseiroa LB, Rauter AP, Mourato Serralheirob ML. Polyphenols as acetylcholinesterase inhibitors: structural specificity and impact on human disease. Nutr Aging, 2012; 1:99-111. https://doi.org/10.3233/NUA-2012-0006

Samudrala PK, Augustine BB, Kasala ER, Bodduluru LN, Barua C, Lahkar M. Evaluation of antitumor activity and antioxidant status of Alternanthera brasiliana against Ehrlich ascites carcinoma in Swiss albino mice. Pharmacogn Res, 2015; 7(1):66-73. https://doi.org/10.4103/0974-8490.147211

Samy RP, Thwin MM, Gopalakrishnakone P, Ignacimuthu S. Ethnobotanical survey of folk plants for the treatment of snakebites in Southern part of Tamilnadu, India. J Ethnopharmacol, 2008; 115(2):302-12. https://doi.org/10.1016/j.jep.2007.10.006

Sathe KN. Gharguti Aushadhe. 16th edition, Shailaja Anil Sathe, Mumbai, India, 2003.

Sells PG, Richards AM, Laing GD, Theakston RDG. The use of hens' eggs as an alternative to the conventional in vivo rodent assay for antidotes to haemorrhagic venoms. Toxicon, 1997; 35(9):1413-21. https://doi.org/10.1016/S0041-0101(97)00022-6

Shashidharamurthy R, Jagadeesha DK, Girish KS, Kemparaju K. Variations in biochemical and pharmacological properties of Indian cobra (Naja naja naja) venom due to geographical distribution. Mol Cell Biochem, 2002; 229(1-2):93-101.

Suntravat M, Nuchprayoon I, Pérez JC. Comparative study of anticoagulant and procoagulant properties of 28 snake venoms from families Elapidae, Viperidae, and purified Russell's viper venom-factor X activator (RVV-X). Toxicon, 2010; 56:544-53. https://doi.org/10.1016/j.toxicon.2010.05.012

Suraweera W, Warrell D, Whitaker R, Menon G, Rodrigues R, Hang Fu S, Begum R, Sati P, Piyasena K, Bhatia M, Brown P, Jha P. Trends in snakebite deaths in India from 2000 to 2019 in a nationally representative mortality study. ELife, 2020; 9:e54076. https://doi.org/10.7554/eLife.54076

Tessou KZ, Lawson-Evi P, Metowogo K, Diallo A, Eklu- Gadegkeku K, Aklikokou K, Gbeassor M. Acute and sub-acute toxicity studies of Plumeria alba Linn. (Apocynaceae) hydroalcoholic extract in rat. Int J Biomed Sci, 2013; 9(4):255-9.

Theakston RDG, Reid HA. Development of simple standard assay procedures for the characterization of snake venoms. Bull World Health Organ, 1983; 61(6):949-56.

Ticli FK, Hage LI, Cambraia RS, Pereira PS, Magro AJ, Fontes MR, Stábeli RG, Giglio JR, França SC, Soares AM, Sampaio SV. Rosmarinic acid, a new snake venom phospholipase A2 inhibitor from Cordia verbenacea (Boraginaceae): antiserum action potentiation and molecular interaction. Toxicon, 2005; 46:318-27. https://doi.org/10.1016/j.toxicon.2005.04.023

Türkan F, Taslimi P, Saltan FZ. Tannic acid as a natural antioxidant compound: discovery of a potent metabolic enzyme inhibitor for a new therapeutic approach in diabetes and Alzheimer's disease. J Biochem Mol Toxicol, 2019; 33:e22340. https://doi.org/10.1002/jbt.22340

Vijayalakshmi A, Ravichandiran V, Velraj M, Hemalatha S, Sudharani G, Jayakumari S. Anti-anaphylactic and anti-inflammatory activities of a bioactive alkaloid from the root bark of Plumeria acutifolia Poir. Asian Pac J Trop Biomed, 2011; 1(5):401-5. https://doi.org/10.1016/S2221-1691(11)60088-9

Whitaker R. Common Indian snakes-a field guide. Macmillan Publishers India Ltd, Chennai, India, 2006.

WHO. Snakebite envenoming. World Health Organization, Geneva, Switerzland, 2021. Available via https://www.who.int/news-room/ fact-sheets/detail/snakebite-envenoming. (Accessed 5 September 2021).

Williams DJ, Abul Faiz M, Abela-Ridder B, Ainsworth S, Bulfone TC, Nickerson AD, Habib AG, Junghanss T, Fan HW, Turner M, Harrison RA, Warrell DA. Strategy for a globally coordinated response to a priority neglected tropical disease: snakebite envenoming. PLOS Negl Trop Dis, 2019; 13(2):e0007059. https://doi.org/10.1371/journal.pntd.0007059

Zhang Y, Li CM. The detoxifying effects of structural elements of persimmon tannin on Chinese cobra phospholipase A2 correlated with their structural disturbing effects well. J Food Drug Anal, 2017; 25:731-40. https://doi.org/10.1016/j.jfda.2016.08.005

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