Laboratory optimization of the production of guanidium-based inactivated transport media to support SARS-CoV-2 PCR testing in Indonesia

Shabarni Gaffar,  Hesti Lina Wiraswati,  Savira Ekawardhani,  Lia Faridah,  Nisa Fauziah,  Amila Laelalugina,  Annisa Retno Arimdayu,  Chanda Taruna Adiarka Utomo,  Maulana Ihsan,  Ikhwan Ramadhana,  Sanny Hafidhoh Siti Nururrohmah,  Trifanny Nur Azizah,  Mia Tria Novianti,  Radewi Safira Fauzia   
Shabarni Gaffar1&2, Hesti Lina Wiraswati3&4&5, Savira Ekawardhani3&4&5, Lia Faridah3&4&5, Nisa Fauziah3&4&5, Amila Laelalugina3, Annisa Retno Arimdayu3, Chanda Taruna Adiarka Utomo3, Maulana Ihsan3, Ikhwan Ramadhana3, Sanny Hafidhoh Siti Nururrohmah3, Trifanny Nur Azizah3, Mia Tria Novianti2&3, Radewi Safira Fauzia3

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Bandung, Indonesia.

2Research Center for Biotechnology and Bioinformatics, Universitas Padjadjaran, Bandung, Indonesia.

3Parasitology Laboratory, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.

4Parasitology Division, Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.

5Infection Study Center, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.

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Published:  Jun 12, 2022

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Abstract

The availability of a viral transport medium (VTM) is often an obstacle to COVID-19 testing, especially for countries that are not VTM producers, including Indonesia. Therefore, the Parasitology Laboratory of Medicinal Faculty, Universitas Padjadjaran, Indonesia, developed an inactivation transport medium called “VITPAD®” that uses a guanidium-based buffer solution. This study evaluated VITPAD® as a transport medium for swab samples and scaled-up production from 2,000 tubes per month to 60,000 per month to support COVID-19 testing in Indonesia. VITPAD® has good stability and sterility, with a lifespan of over 2 years, and it performs well as a transport medium for COVID-19 samples. The production capacity was successfully scaled-up by implementing scheduling and capacity management, with the first large-scale production of VITPAD® distributed to 26 teaching hospitals in Indonesia.


Keyword:     Guanidium-based buffer inactivation transport media polymerase chain reaction production capacity.

Citation:

Gaffar S, Wiraswati HL, Ekawardhani S, Faridah L, Fauziah N, Laelalugina A, Arimdayu AR, Utomo CTA, Ihsan M, Ramadhana I, Nururrohmah SHS, Azizah TN, Novianti MT, Fauzia RS. Laboratory optimization of the production of guanidium-based inactivated transport media to support SARS-CoV-2 PCR testing in Indonesia. J Appl Pharm Sci,2022. Online First.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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