Research Article | Volume: 11, Supplement 1, March, 2021

Effect of abiotic factors on bacoside A content, acetylcholinesterase inhibitory and antioxidant activities of Bacopa monnieri (L.) Wettst

Varinder Singh Naman Jain Richa Shri   

Open Access   

Published:  Feb 11, 2021

DOI: 10.7324/JAPS.2021.11s107

Growth of plants and production of phytoconstituents are influenced by abiotic stresses. Understanding these abiotic factors and their subsequent modification during cultivation/growth of medicinal plants may help in increasing the production of valuable secondary metabolites. The present study examined the effect of various abiotic factors and seasons on the growth of Bacopa monnieri, production of the marker compound bacoside A, and antioxidant and acetylcholinestrase inhibitory activities of the plant extract. The plant was cultivated in two different soils, viz A (clay loam soil) and B (sandy loam soil). Different abiotic stresses, i.e., water stress, fertilization, light, and salinity, were applied in two seasons (season 1: July to November and season 2: February to May). Bacoside A content in methanol extracts of the dried aerial parts of plants grown under different stresses was determined using a validated thin layer chromatography (TLC) densitometric method. In vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and Ellman’s method were employed to evaluate the antioxidant and acetylcholinestrase inhibitory activities of different extracts, respectively. Best plant growth was observed in soil A in season 1. Plants grown in season 2 had a significantly higher bacoside A content, better antioxidant, and acetylcholinestrase inhibitory activities than the plants grown in season 1. Among various stresses applied in season 2, plants grown in soil B, especially water stress-effected plants, had the highest bacoside A content, antioxidant, and acetylcholinestrase inhibitory activities. The plant should be cultivated from February to Ma

Keyword:     Abiotic stresses acetylcholinesterase inhibition antioxidant activity Bacopa monnieri bacoside A.


Singh V, Jain N, Shri R. Effect of abiotic factors on bacoside A content, acetylcholinesterase inhibitory and antioxidant activities of Bacopa monnieri (L.) Wettst. J Appl Pharm Sci, 2021; 11 (Supp 1):063–070.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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