Research Article | Volume: 9, Supplement 1, March, 2019

Targeted metabolomics reveals the therapeutic impact of Eclipta prostrata on diet-induced non-alcoholic fatty liver disease in rats

Azza S. Helmy Naglaa M. Sherif Hassan Z. Ghanem Nabaweya A. Ibrahim Abdel Nasser G. El Gendy Noha S. Hussein Abdel-Hamid Z. Abdel-Hamid   

Open Access   

Published:  Mar 10, 2019

DOI: 10.7324/JAPS.2019.S109
Abstract

Non-alcoholic fatty liver disease (NAFLD) is a growing clinical problem worldwide. The current study aimed to evaluate the therapeutic effect of the methanol extract of Eclipta prostrata (E. prostrata) in rats using gas chromatography–mass spectrometry (GC–MS) followed by multivariate and univariate data analysis. Phytochemical study of E. prostrata extract was performed using chemical, colorimetric, and high-performance liquid chromatography (HPLC) analysis. Forty-two Wistar albino rats divided into seven groups (six in each) were used in this study. Fatty liver was induced in rats using a high-fat diet together with cholesterol and cholic acid, then they were treated with different doses of E. prostrata. The histopathological examination of liver and NAFLD activity score showed significant markers for the diagnosis of the early stage of the disease. Treatment with high doses of E. prostrata (300 and 200 mg/kg BW) extract exhibited significant improvement in liver function and lipid profile in rats bearing NAFLD. Lipidomic analysis showed a discrete variability in the levels of some metabolites, including cholest-5-en-3-ol (3β) – acetate; cholesta-4, 6-dien-3-ol, (3β); urea; 1,3 dipalmitin; glycerol; linoleic acid; and arachidonic acid in sera of rats bearing NAFLD in contrast to healthy ones. Treatment with E. prostrata extract showed noticeable amelioration in the levels of these metabolites. The present results concluded that E. prostrata in high doses (300 and 200 mg/kg BW) had the potential efficacy to ameliorate NAFLD. Nevertheless, lipidomics could be used for the early detection of NAFLD and in the assessment of the mechanism of herbal medications.


Keyword:     Eclipta prostrata GC–MS Lipidomics NAFLD.


Citation:

Helmy AS, Sherif NM, Ghanem HZ, Ibrahim NA, El Gendy AG, Hussein NS, Abdel-Hamid AZ. Targeted metabolomics reveals the therapeutic impact of Eclipta prostrata on diet-induced non-alcoholic fatty liver disease in rats. J Appl Pharm Sci, 2019; 9(S1):077–090.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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