Research Article | Volume: 9, Supplement 1, March, 2019

ZnO nanoparticles: Crosslink between cytotoxicity on liver cell lines and In Vivo biosafety on different mice organs

Nahla Nabil Kamel Maha Zaki Rizk Walaa Gamal Hozayen Abdel-Hamid Zaki Abdel-Hamid   

Open Access   

Published:  Mar 10, 2019

DOI: 10.7324/JAPS.2019.S107

The wide applications of zinc oxide nanoparticles (ZnO NPs) in several biomedical aspects have raised many concerns about their toxicity effects. This study aimed to explore the cytotoxic effect of different sizes (14 , 30, and 50 nm) of ZnO NPs on liver cancer cell lines hepatoma G2 (HepG2), HuH7 cells (HuH7 hepatoma cells) versus normal cells (THLE2 cells). Cytotoxicity, oxidative stress, and apoptosis were investigated. The results pointed out that the particle size of 14 nm recorded the highest activity, whereas no cytotoxic effect was observed on THLE2. Also, oxidative stress elicited a reduction in reduced glutathione with an increase in lipid peroxides and caspase-3. In addition, RT-PCR revealed a significant up-regulation in caspase-3 gene expression. Histopathological investigation confirmed the biosafety of the same particle size which revealed the least toxic effect on all mice organs (liver, kidney, lung, and brain). In conclusion, this particle size of ZnO NPs could be useful in future therapeutic applications on liver cancer.

Keyword:     ZnO nanoparticles HepG2 cells HuH7 liver cancer cytotoxicity.


Kamel NN, Rizk MZ, Hozayen WG, Abdel-Hamid A-HZ. ZnO nanoparticles: Crosslink between cytotoxicity on liver cell lines and In Vivo biosafety on different mice organs. J Appl Pharm Sci, 2019; 9(S1):058–066.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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