Research Article | Volume: 9, Supplement 1, March, 2019

Cressa cretica L. growing in Egypt: Phytochemical study and potential antioxidant and hepato-protective activities

Taha S. El-Alfy Nagwa M. Ammar Sahar Y Al-Okbi Maha M Salama Hanan F. Aly Asmaa A Amer   

Open Access   

Published:  Mar 10, 2019

DOI: 10.7324/JAPS.2019.S106
Abstract

The present research was conducted to study the phytochemical constituents of the successive extracts of aerial parts of Cressa cretica L. and their antioxidant and hepatoprotective activities. Phenolic and flavonoid contents and their respective compounds were determined in ethyl acetate and 70% ethanol extract using chromatographic and spectral analyses. Unsaponifiable matter (USM) and fatty acids were investigated in the lipoidal matter by gas chromatography–mass spectrometry analysis. In vitro 2,2-diphenyl-1-picryl-hydrazyl radical scavenging activity and safety of successive extracts were assessed. The hepatoprotective effect of the safest extracts was evaluated in CCl4 intoxicated rats. Results showed that total phenolic contents were 21.07 and 22.95 mg Gallic aid equivalent/g in ethyl acetate and 70% ethanol extract, respectively, while total flavonoid contents were 6.25 and 10.18 mg catechin equivalent/g, respectively. Catechin and chlorogenic acid were the major phenolic compounds in ethyl acetate and ethanol extract, while rutin and apigenin-7-glucoside were the predominant flavonoids, respectively. Phytol was the major compound in USM, while 14-methyl-pentadecanoic acid was the predominant fatty acid. The most promising antioxidant was 70% ethanol extract. Ethyl acetate, 70% ethanol, and petroleum ether extracts showed complete safety and produced a hepatoprotective effect; ethyl acetate and 70% ethanol extracts were superior and comparable to silymarin and even more potent concerning specific biomarkers and histopathology.


Keyword:     Cressa cretica L. bioactive fractions hepatotoxicity antioxidant activity.


Citation:

El-Alfy TS, Ammar NM, Al-Okbi SY, Salama MM, Aly HF, Amer AA. Cressa cretica. L. growing in Egypt: Phytochemical study and potential antioxidant and hepato-protective activities. J Appl Pharm Sci, 2019; 9(S1):046–057.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Adams RP. Identification of essential oil components by gas chromatography mass spectroscopy. Allured Publishing Corporation, Carol Stream, IL, 1995.

Al-Okbi SY, Abdel-Razek AG, Mohammed SE, Ottai ME. Roselle seed as a potential new source of healthy edible oil. J Biol Sci, 2017; 17(6):267–77. https://doi.org/10.3923/jbs.2017.267.277

Al-Okbi SY, Mohamed DA, Hamed TE, Edris AE, Fouda K. Hepatic regeneration and reno-protection by fish oil, Nigella sativa oil and combined fish oil/Nigella sativa volatiles in CCl4 treated rats. J Oleo Sci, 2018; 67(3):345–53. https://doi.org/10.5650/jos.ess17204

Al-Olayan EM, El-Khadragy MF, Metwally DM, A bdelMoneim AE. Protective effects of pomegranate (Punica granatum) juice on testes against carbon tetrachloride intoxication in rats. BMC Complem Altern Med, 2014; 14:1–9. https://doi.org/10.1186/1472-6882-14-164

Ammar NM, Hefnawy MS, Al-Okbi SY, Mohamed DA, El- Sayed NK, El-Anssary AA, Mabry T. Phytochemical and biological studies of Butia capitata Becc. Leaves cultivated in Egypt. Asian Pac J Trop Biomed, 2014; 4(6):456–62. https://doi.org/10.12980/APJTB.4.2014C1192

Anguo W, Tingting D, Dan T, Youhua X, Liang F, Zhaoguang Z, Jiaxiao Z, Rushang W, Quan Z. Determination of nitric oxide-derived nitrite and nitrate in biological samples by HPLC coupled to nitrite oxidation. Chromatographia, 2013; 76:1649–55. https://doi.org/10.1007/s10337-013-2529-0

Bahar A. Cresoside: a new coumaranochromone glycoside from fruits of Cressa cretica Linn. Indian J Nat Prod, 1998; 14(2):29–32.

Bergmeyer H, Bernt E. Practical clinical biochemistry. 5th edition, Willian Heinmann Medical Books Ltd, London, UK, p 741, 1980.

Bird RP, Draper HH. Effect of malonaldehyde and acetaldehyde on cultured mammalian cells: growth, morphology, and synthesis of macromolecules. J Toxicol Environ Health, 1980; 6:811–23. https://doi.org/10.1080/15287398009529899

Boulos L. Flora of Egypt checklist. Al-Hadara Publishing, Cairo, Egypt, p 174, 2009.

Bruckner J. Estimation of the direct and total bilirubin in serum investigations and observations by a modified method. Clin Chim Acta, 1961; 6:370–5. https://doi.org/10.1016/0009-8981(61)90064-X

Buege JA, Aust SD. Microsomal lipid, peroxidation. In: Flesicher S, Packer L (eds.). Methods in enzymology. Academic Press, New-York, 52, p 302–10, 1978. https://doi.org/10.1016/S0076-6879(78)52032-6

Chapman HD, Pratt PT. Methods of analysis for soils, plants and water. University of California, Dept. of Agric. Sci, California, p 320, 1978.

Dash DK, Yeligar VC, Nayak SS, Ghosh T, Rajalingam R, Sengupta P, Maiti BC, Maity TK. Evaluation of hepatoprotective and antioxidant activity of Ichnocarpus frutescens (Linn.) R. Br. on paracetamol-induced hepatotoxicity in rats. Trop J Pharm Res, 2007; 6:755–65. https://doi.org/10.4314/tjpr.v6i3.14656

El-Hawary SA, Sokkar NM, Ali ZY, Yehia MM. A profile of bio¬active compounds of Rumex vesicarius L. J Food Sci, 2011; 76:C1195–202. https://doi.org/10.1111/j.1750-3841.2011.02370.x

Finar IL. Organic chemistry. 5th edition, Longmans Green and Co. Ltd, London, UK, Vol. 1, p 212, 1967.

Gülçin IU, Oktay M, KüfrevioÄŸlu ÖI, Aslan A. Determination of antioxidant activity of lichen Cetrariaislandica (L.), Ach. J Ethnopharmacol, 2002; 79:325–9. https://doi.org/10.1016/S0378-8741(01)00396-8

Ha BJ, Lee JY. The effect of chondroitin sulfate against CCl4- induced hepatotoxicity. Biol Pharm Bull, 2003; 26:622–6. https://doi.org/10.1248/bpb.26.622

Hirsch MS, Faquin WC, Krane JF. Thyroid transcription factor-1, but not p53, is helpful in distinguishing moderately differentiated neuroendocrine carcinoma of the larynx from medullary carcinoma of the thyroid. Mod Pathol, 2004; 17:631–6. https://doi.org/10.1038/modpathol.3800105

Hussain S, Ahmed E, Malik A, Jabbar A, Arshad M. Phytochemical studies of Cressacretica. J Chem Soc Pak, 2005; 27(3):296–8.

Islam MT, de Alencar MV, da Conceição Machado K, da Conceição Machado K, de CarvalhoMelo-Cavalcante AA, de Sousa DP, de Freitas RM. Phytol in a pharma-medico-stance. Chem Biol Interact, 2015; 240:60–73. https://doi.org/10.1016/j.cbi.2015.07.010

Khan RA, Khan MR, Sahreen S. CCl4-induced hepatotoxicity: protective effect of rutin on p53, CYP2E1 and the antioxidative status in rat. BMC Complem Altern Med, 2012; 8:172–8. https://doi.org/10.1186/1472-6882-12-178

Kim K, Tsao R, Yang R, Cui, SW. Phenolic acid profiles and antioxidant activities of wheat bran extracts and the effect of hydrolysis conditions. Food Chem, 2006; 95(3):466–73. https://doi.org/10.1016/j.foodchem.2005.01.032

Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clinic Pathol, 2001; 54:356–61. https://doi.org/10.1136/jcp.54.5.356

Kummar PV, Sivarag A, Elumalai EK, Kumar BS. Carbon tetrachloride-induced hepatotoxicity in rats-Protective role of aqueous leaf extracts of Coccinia grandis. Int J Pharm Tech Res CODEN (USA), 2009; 1(4):1612–5.

Kwun IS, Jang HS, Kwon CS. Plasma level of antioxidant minerals (Cu, Zn, Mn, and Se) and Fe. In: Roussel AM, Anderson RA, Favrier AE (eds.). Trace elements in man and animals. Springer, Boston, MA, 2002. https://doi.org/10.1007/0-306-47466-2_178

Lindsay WL, Norvell WA. Development of a DTPA micronutrient soil tests for Zinc, iron, manganese and copper. J Soil Sci Amer, 1978; 42:421–8. https://doi.org/10.2136/sssaj1978.03615995004200030009x

Lou Z, Minter-Dykhouse K, Wu X, Chen J. MDC1 is coupled to activate CHK2 in mammalian DNA damage response pathways. Nature, 2003; 421:957–61. https://doi.org/10.1038/nature01447

Lukaszewicz HJ, Moniuszko J. Liver catalase, glutathione peroxidase and reductase activity, reduced glutathione and hydrogen peroxide levels in acute intoxication with chlorfenvinphos, an organophosphate insecticide A. Pol J Environ Stud, 2004; 13:303–9.

Mabberley DJ. The Plant-book. CPI Group Ltd, Croydon, UK, Macdonald HG. A dictionary of natural products. N.J.7 Plexus Publishing, Medford, p 187, 1997.

Madhu KP, Vijaya Raju A, Ganga Rao B. Investigation of hepatoprotective activity of Cyatheagigantea (Wall. ex. Hook.) leaves against paracetamol–induced hepatotoxicity in rats. Asian Pac J Trop Biomed, 2012; 2(5):352–6. https://doi.org/10.1016/S2221-1691(12)60055-0

Marsillach J, Camps J, Ferre N, Beltran R, Rul A, Mackness B, Michael M, Jorge J. Paraoxonase-1 is related to inflammation, fibrosis and PPAR delta in experimental liver disease. BMC Gastroenterol, 2009; 9:1–13. https://doi.org/10.1186/1471-230X-9-3

Mohamed NZ, Abd-Alla HI, Aly HF, Mantawy M, Ibrahim N, Hassan SA. CCl4-induced hepatonephrotoxicity: protective effect of nutraceuticals on inflammatory factors and antioxidative status in rat. J Appl Pharm Sci, 2014; 4:087–100.

Moron MS, Depierre JW, Mannervik B. Level of glutathione, glutathione reductase and glutathone-S-transferase activities in rat lung and liver. Biochem Biophys Acta, 1979; 582:67–78. https://doi.org/10.1016/0304-4165(79)90289-7

Mohamed DA, Hamed TE, Al-Okbi SY. Reduction of hypercholesterolemia and risk of cardiovascular diseases by plant foods extracts mixtures: a study on plasma lipid profile, oxidative stress and testosterone in rats. Grasas y Aceites, 2010; 61(4):378–89. https://doi.org/10.3989/gya.021210

Nishikimi M, Rae NA, Yagi K. The occurrence of superoxide anion in the action of reduced phenazinemethosulphate and molecular oxygen. Biochem Biophys Res Commun, 1972; 46:849–53. https://doi.org/10.1016/S0006-291X(72)80218-3

Oktay M, Gülçin I, Küfrevio I. Determination of in vitro antioxidant activity of fennel (Foeniculumvulgare) seed extracts. Lebensm- Wiss Technol, 2003; 36:263–71. https://doi.org/10.1016/S0023-6438(02)00226-8

Palanivel MG, Rajkapoor B, Kumar RS, EInstein J W, Kumar EP, Kumar MR, Kavitha K, Kumar MP, Jayakar B. Hepatoprotective and antioxidant effect of Pisoniaaculeata L. against CCl4-induced hepatic damage in rats. Sci Pharm, 2008; 76:203–15. https://doi.org/10.3797/scipharm.0803-16

Parmar SR, Patel HV, Kalia K. Hepatoprotective activity of some plants extract against paracetamol induced hepatotoxicity in Wistar rats. J Herbal Med Toxicol, 2010; 4(2):101–6.

Peng WH, Chen YW, Lee MS, Chang WT, Tsai JC, Lin YCh, Lin MK. Hepatoprotective effect of Cuscutacampestris Yunck. whole plant on carbon tetrachloride induced chronic liver injury in mice. Int J Mol Sci, 2016; 17(12):2056. https://doi.org/10.3390/ijms17122056

Prakash J, Gupta SK, Kochupillai V, Singh N, Gupta YK, Joshi S. Chemopreventive activity of Withaniasomnifera in experimentally induced fibrosarcomatumours in swiss albino mice. Phytother Res, 2001; 15:240–4. https://doi.org/10.1002/ptr.779

Priyashree S, Jha S, Pattanayak SP. A review on Cressa cretica Linn.: a halophytic plant. Pharmacogn Rev, 2010; 4(8):161–6. https://doi.org/10.4103/0973-7847.70910

Raje S, Sane RT, Mangaonkar K, Shailajan S, Pathak G, Jariwala N, Kasar D. Determination of heavy metals from Cressa cretica using atomic absorption spectroscopic technique. J Indian Chem Soc, 2006; 83(6):611–2.

Ramachandran R, Ali M. Isolation and characterization of acyclic terpenic constituents from Cressa cretica aerial parts. J Med Aromat Plant Sci, 2003; 25(1):81–90.

Rastogi RP, Mehrotra BN. Compendium of Indian medicinal plants. Central Drug Research Institute, Lucknow and National institute of science communication and information resources, New Delhi, Vol. I, p 126, 2004.

Rastogi RP, Mehrotra BN. Compendium of Indian medicinal plants. Central Drug Research Institute, Lucknow and National institute of science communication and information resources, New Delhi, Vol. III, p 254, 2005.

Reitman S, Frankel S. Colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Path, 1957; 28:56–63. https://doi.org/10.1093/ajcp/28.1.56

Romano CS, Abadi K, Repetto V, Vojnov AA, Moreno S. Synergistic antioxidant and antibacterial activity of rosemary plus butylated derivatives. Food Chem, 2009; 115:456–61. https://doi.org/10.1016/j.foodchem.2008.12.029

Sahreen S, Khan MR, Khan RA. Hepatoprotective effects of methanol extract of Carissa opaca leaves on CCl4-induced damage in rat. BMC Complem Altern Med, 2011; 11:48. https://doi.org/10.1186/1472-6882-11-48

Satakopan S, Karandikar GK. Studies in the American convolvulaceae. J Sci Ind Res, 1961; 20:156.

Shahat AA, Abdel-Azim NS, Pieters L, Vlietinck AJ. Isolation and NMR spectra of syringaresinol-h-dglucoside from Cressacretica, Fitoterapia, 2004; 75(7–8):771–3. https://doi.org/10.1016/j.fitote.2004.05.008

Shahat AA, Nazif NM, Nahla AS, Luc P, Arnold VJ. Flavonoids from Cressa Cretica. Qatar Uni Sci J, 2005; 25:72–7.

Sharma RK, Siddiqi JN, Sharma B. Protective effect of Silymarin on human erythrocyte against tert-butyl hydroperoxide induced oxidative stress in vitro. Am J Biochem Mol Biol, 2012; 2:167–74. https://doi.org/10.3923/ajbmb.2012.167.174

Silva RO, Sousa FB, Damasceno SR, Carvalho NS, Silva VG, Oliveira FR, Sousa DP, Aragão KS, Barbosa AL, Freitas RM, Medeiros JV. Phytol, a diterpene alcohol, inhibits the inflammatory response by reducing cytokine production and oxidative stress. Fundam Clin Pharmacol, 2014; 28(4):455–64. https://doi.org/10.1111/fcp.12049

Sunita P, Jha S, Pattanayak SP. Anti-inflammatory and in-vivo antioxidant activities of Cressa cretica Linn., a Halophytic plant. Middle- East J Sci Res, 2011; 8(1):129–40.

Täckholm V. Students' flora of Egypt. 2nd edition, Cairo University Press, Cairo, Egypt, 1974.

Thirunavukkarasu, P, Asha S, Ramanathan T, Balasubramanian T, Shanmogapriya R, Renugadevi G. In vitro hepatoprotective activity of isolated fractions of Cressa Cretica. Pharm Chem J, 2014; 48(2):121–6. https://doi.org/10.1007/s11094-014-1061-3

Tsuda K, Sakai K, Tanabe K, Kishida Y. Isolation of 22-dehydrocholestrol from Hypnea japonica. J Am Chem Soc, 1960; 82:1442–3. https://doi.org/10.1021/ja01491a040

Zilic S, Serpen A, Akillioglu G, Jankovic M, Gokmen V. Distributions of phenolic compounds, yellow pigments and oxidative enzymes in wheat grains and their relation to antioxidant capacity of bran and debranned flour. J Cereal Sci, 2012; 56:652–8. https://doi.org/10.1016/j.jcs.2012.07.014

Article Metrics

434 Absract views 43 PDF Downloads 477 Total views

   Abstract      Pdf Download

Related Search

By author names

Citiaion Alert By Google Scholar

Name Required
Email Required Invalid Email Address

Comment required