Research Article | Volume: 9, Supplement 1, March, 2019

Detoxification and anti-mycotoxigenic effect of Ochratoxin A produced by Penicillium expansum species utilizing probiotic Pichia kudriavzevii and biogenic-selenium nano-formulations

Eman Ahmed Mohamed Helmy   

Open Access   

Published:  Mar 08, 2019

DOI: 10.7324/JAPS.2019.S101
Abstract

Ochratoxins (OT) are overall spread fungal secondary metabolites combined chiefly by some toxigenic types of Aspergillus and Penicillium. A few physical, chemical, and biological techniques were found among literary reports to wipe out, inactivate, or decrease the bioavailability of mycotoxins counting ochratoxin A (OTA). The aim of this study was to evaluate the combined inhibitory capacity of selenium nanoparticles (SeNPs) and probiotic yeast Pichia kudriavzevii on OTA production by seven mycotoxigenic Penicillium expansum strains. The detoxification capability of OTA was tested using thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) techniques. The mixture of the probiotic P. kudriavzevii and SeNPs demonstrated that most of OTA reduction rate created by P. expansum5 by a rate of (58.38%). While the least OTA removal rate was delivered by P. expansum3 by (34.47%) treated with SeNPs as it were. The ultrastructural investigations of P. expansum5 cells completed with transmission electron microscopy demonstrated among the cytoplasmic constituents of P. expansum5 cells treated with the previous mix of probiotic and SeNPs that were totally contracted and pulled back far from the cell membrane leaving a gigantic separation region of no organelles. Remarkable cytoplasmic ultra-structure’s deformations of cells were additionally recorded accompanied with a vacuolation as a survival adaptation mode of fungal cells. Conclusively, a strategy of combined probiotic yeast P. kudriavzevii and biogenic-SeNPs is useful as natural blends for detoxification, anti-mycotoxigenic, and/or reduction of OTA production by mycotoxigenic fungal species of P. expansum. This natural mixture could be recommended as food/feed preservative and as a prophylactic agent in pre- and post-harvest crops.


Keyword:     OTA Detoxification Probiotic Pichia kudriavzevii Biogenic-Selenium Nano.


Citation:

Helmy EAM. Detoxification and anti-mycotoxigenic of Ochratoxin A produced by Penicillium expansum species utilizing probiotic Pichia kudriavzevii and biogenic-selenium nano-formulations. J Appl Pharm Sci, 2019; 9(S1): 001–008.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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