Antibacterial and antibiofilm activity of cinnamaldehyde against carbapenem-resistant Acinetobacter baumannii in Egypt: In vitro study

Sara H. Mohamed; Dalia Salem; Magda Azmy; Nevine S. Fam

doi:10.7324/JAPS.2018.81121

Abstract References Article Metrics Similar Articles Request Permission Related Search Citation Alert By Google Scholar Comment On This Article

Abstract

Acinetobacter baumannii has emerged as a problematic nosocomial pathogen due to its antibiotic resistance as well as its ability to colonize and cause serious infection among patients. This study aimed to evaluate the ability of A. baumannii to form biofilms as well as to investigate the antibacterial activity of cinnamaldehyde against carbapenem-resistant strains of A. baumannii. A total of 23 A. baumannii clinical strains were screened for their ability to form a biofilm using tissue culture plate method. Cinnamaldehyde antibacterial ability was investigated on planktonic cells and its biofilm inhibition ability was tested. Scanning electron microscopy (SEM) was applied to confirm the antibiofilm effect of cinnamaldehyde. Biofilm formers (86.95%) were categorized into strong (17.39%), moderate (52.17%), and weak (17.39%). Cinnamaldehyde showed a strong antimicrobial activity against planktonic cells of A. baumannii at low concentrations. The best antibiofilm activity was achieved at ½ minimum inhibitory concentration (MIC) and ¼ MIC causing inhibition percentages ranging from 49.5% to 71.2% and 18.5% to 29.6%, respectively. Cinnamaldehyde exerted strong antimicrobial and antibiofilm properties indicating their potential therapeutic value that can be used as an option for treating biofilm associated clinical problems caused by A. baumannii.

Keyword: Acinetobacter baumannii biofilm carbapenem-resistant cinnamaldehyde SEM.

Citation:

Mohamed SH, Salem D, Azmy M, Fam NS. Antibacterial and antibiofilm activity of cinnamaldehyde against carbapenemresistant Acinetobacter baumannii in Egypt: In vitro study. J App Pharm Sci, 2018; 8(11): 151–156.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Abdulhasan GA, Alzubaidy SK, Abed IJ. Effect of sub-inhibitory and inhibitory concentrations of some antibiotics and rosemary essential oil (Rosmarinus officinalis L.) on biofilm formation of Klebsiella pneumoniae. World J Exp Biosci, 2016; 4:130–5.
Adukwu EC, Allen SCH, Phillips CA. The anti-biofilm activity of lemongrass (Cymbopogon flexuosus) and grapefruit (Citrus paradisi) essential oils against five strains of Staphylococcus aureus. J Appl Microbiol, 2012; 113:1217–27.https://doi.org/10.1111/j.1365-2672.2012.05418.x
Almaghrabi MK, Joseph MRP, Assiry MM, Hamid ME. Multidrug-resistant Acinetobacter baumannii: an emerging health threat in Aseer Region, Kingdom of Saudi Arabia. Can J Infect Dis Med Microbiol, 2018; 2018:9182747; doi:10.1155/2018/9182747https://doi.org/10.1155/2018/9182747
Anish C, Abhisek R, Radha M. Evaluation of biofilm production in Acinetobacter baumanii with reference to imipenem resistance. Inter J Sci Res Pub, 2017; 7:732–7.
Authman SH, Ali FS, Marjani MFA. Biofilm formation in imipenem-resistant Acinetobacter baumannii from the intensive care unit. J Glob Pharma Technol, 2017; 10:404–11.
Azizun N, Shaheda A, Miah MRA. Association of biofilm formation with antimicrobial resistance among the Acinetobacter species in a tertiary care hospital in Bangladesh. J Med, 2013; 14:28–32.https://doi.org/10.3329/jom.v14i1.14533
Badave GK, Dhananjay K. Biofilm producing multidrug resistant Acinetobacter baumannii: an emerging challenge. J Clin Diagnostic Res, 2015; 9:DC08–10.https://doi.org/10.7860/JCDR/2015/11014.5398
Bae S, Kim MC, Park SJ,Kim HS, Sung H, Kim MN, Kim SH, Lee SO, Choi SH, Woo JH, Kim YS, Chong YP. In vitro synergistic activity of antimicrobial agents in combination against clinical isolates of colistin-resistant Acinetobacter baumannii. Antimicrob Agents Chemother, 2016; 60:6774–9.https://doi.org/10.1128/AAC.00839-16
Bai AJ, Vittal RR. Quorum sensing inhibitory and anti-Biofilm activity of essential oils and their in vivo efficacy in food systems. Food Biotechnol, 2014; 28:269–92.https://doi.org/10.1080/08905436.2014.932287
Bialvaei AZ, Kouhsari E, Salehi-Abargouei A, Amirmozafari N, Ramazanzadeh R, Ghadimi-Daresajini A, Sedighi M. Epidemiology of multidrug-resistant Acinetobacter baumannii strains in Iran: a systematic review and meta-analysis. J Chemother, 2017; 29:327–37.https://doi.org/10.1080/1120009X.2017.1338377
Bogdan M, Drenjancevic D, Harsanji Drenjancevic I, Bedenic B, Zujic Atalic V, Talapko J, Vukovic D. In vitro effect of subminimal inhibitory concentrations of antibiotics on the biofilm formation ability of Acinetobacter baumannii clinical isolates. J Chemother, 2017; 9478:1–9.
BudzyÅ„ska A, Wieckowska-Szakiel M, Sadowska B, Kalemba D, Rózalska B. Antibiofilm activity of selected plant essential oils and their major components. Pol J Microbiol, 2011; 60:35–41.
De Campos PA, Royer S, da Fonseca Batistão DW, Araújo BF, Queiroz LL, de Brito CS, Gontijo-Filho PP, Ribas RM. Multidrug resistance related to biofilm formation in Acinetobacter baumannii and Klebsiella pneumoniae clinical strains from different pulsotypes. Curr Microbiol, 2016; 72:617–27.https://doi.org/10.1007/s00284-016-0996-x
Dahdouh E, Hajjar M, Suarez M, Daoud Z. Acinetobacter baumannii isolated from Lebanese patients: phenotypes and genotypes of resistance, clonality, and determinants of pathogenicity. Front Cell Infect Microbiol, 2016; 6:1–10.https://doi.org/10.3389/fcimb.2016.00163
Dehbalaei MA, Najar-Peerayeh S, Taherikalani M, Behmanesh M. Clinical isolates of Acinetobacter baumannii from tehran hospitals: pulsed-field gel electrophoresis characterization, clonal lineages, antibiotic susceptibility, and biofilm-forming ability. Jundishapur J Microbiol, 2017; 10:e13790; doi:10.5812/jjm.13790https://doi.org/10.5812/jjm.13790
El-Kady RA. Acinetobacter baumannii : correlation between biofilm production and multidrug resistance. Int J Adv Res, 2015; 3:691–9.
Elhabibi T, Ramzy S. Biofilm production by multi drug resistant bacterial pathogens isolated from patients in intensive care units in Egyptian hospitals. J Microb Biochem Technol, 2017; 09:151–8.
Fam N, Gamal D, Azmy M, Gamal D. Antimicrobial efficacy of doripenem colistin combination on carbapenem-resistant Acinetobacter baumanii isolates by E-test agar dilution and ultrastrastructural methods. Egypt J Med Microbiol, 2017; 26:1–7.https://doi.org/10.12816/0046266
Ferro TA, Araújo JM, Dos Santos Pinto BL, Dos Santos JS, Souza EB, da Silva BL, Colares VLP, Novais TMG, Filho CMB, Struve C, Calixto JB, Monteiro-Neto V, da Silva LC, Fernandes ES. Cinnamaldehyde inhibits Staphylococcus aureus virulence factors and protects against infection in a Galleria mellonella model. Front Microbiol, 2016; 7:1–10.https://doi.org/10.3389/fmicb.2016.02052
Friedman M. Chemistry, antimicrobial mechanisms, and antibiotic activities of cinnamaldehyde against pathogenic bacteria in animal feeds and human foods. J Agric Food Chem, 2017; 65:10406–23.https://doi.org/10.1021/acs.jafc.7b04344
Gupta PD, Birdi TJ. Development of botanicals to combat antibiotic resistance. J Ayurveda Integr Med, 2017; 8:266–75.https://doi.org/10.1016/j.jaim.2017.05.004
Hu YF, Hou CJY, Kuo CF, Wang NY, Wu AY, Leung CH, Liu CP, Yeh HI. Emergence of carbapenem-resistant Acinetobacter baumannii ST787 in clinical isolates from blood in a tertiary teaching hospital in Northern Taiwan. J Microbiol Immunol Infect, 2017; 50:640–5.https://doi.org/10.1016/j.jmii.2016.08.025
Imane M, Hafida H, Samia B, Ryad D. Biofilm formation by Acinetobacter baumannii isolated from medical devices at the intensive care unit of the University Hospital of Tlemcen (Algeria). Afr J Microbiol Res, 2014; 8:270–6.https://doi.org/10.5897/AJMR2013.6288
IvankoviÄ‡ T, GoiÄ‡-BarišiÄ‡ I, HrenoviÄ‡ J. Reduced susceptibility to disinfectants of Acinetobacter baumannii biofilms on glass and ceramic. Arh Hig Rada Toksikol, 2017; 68:53–8.https://doi.org/10.1515/aiht-2017-68-2946
Jia P, Xue YJ, Duan XJ, Shao SH. Effect of cinnamaldehyde on biofilm formation and sarA expression by methicillin-resistant Staphylococcus aureus. Lett Appl Microbiol, 2011; 53:409–16.https://doi.org/10.1111/j.1472-765X.2011.03122.x
Khan SN, Khan S, Iqbal J, Khan R, Khan AU. Enhanced killing and antibiofilm activity of encapsulated cinnamaldehyde against Candida albicans. Front Microbiol, 2017; 8:1–15.https://doi.org/10.3389/fmicb.2017.01641
Kim YG, Lee JH, Gwon G, Kim SI, Park JG, Lee J. Essential oils and eugenols inhibit biofilm formation and the virulence of Escherichia coli O157:H7. Sci Rep, 2016; 6:1–11.https://doi.org/10.1038/srep36377
Longo F, Vuotto C, Donelli G. Biofilm formation in Acinetobacter baumannii. New Microbiol, 2014; 37:119–27.
Mansour MK, Rhman SAA. Biofilm formation and its association with multiple drug resistance among clinical isolates of Acinetobacter baumanii. Egypt J Med Microbiol, 2012; 21:81–9.https://doi.org/10.12816/0004883
Mohamed SH, Mohamed MSM, Khalil MS, Azmy M, Mabrouk MI. Combination of essential oil and ciprofloxacin to inhibit/eradicate biofilms in multidrug-resistant Klebsiella pneumoniae. J Appl Microbiol, 2018a; 125:84–95.https://doi.org/10.1111/jam.13755
Mohamed SH, Mohamed MSM, Khalil MS, Mohamed WS. Antibiofilm activity of papain enzyme against pathogenic Klebsiella pneumoniae. J Appl Pharm Sci, 2018b; 8:163–8.https://doi.org/10.7324/JAPS.2018.8621
Niu C, Afre S, Gilbert ES. Subinhibitory concentrations of cinnamaldehyde interfere with quorum sensing. Lett Appl Microbiol, 2006; 43:489–94.https://doi.org/10.1111/j.1472-765X.2006.02001.x
Nowotarska S, Nowotarski K, Grant IR, Elliott CT, Friedman M, Situ C. Mechanisms of antimicrobial action of cinnamon and oregano oils, cinnamaldehyde, carvacrol, 2,5-Dihydroxybenzaldehyde, and 2-Hydroxy-5-Methoxybenzaldehyde against Mycobacterium avium subsp. paratuberculosis (Map). Foods, 2017; 6:72.https://doi.org/10.3390/foods6090072
Ramanathan S, Suda KJ, Fitzpatrick MA, Poggensee L, LaVela SL, Burns SP, Evans CT. Multidrug-resistant Acinetobacter: risk factors and outcomes in veterans with spinal cord injuries and disorders. Am J Infect Control, 2017; 45:1183–9.https://doi.org/10.1016/j.ajic.2017.06.016
Reena AAA, Subramaniyan A, Kanungo R. Biofilm formation as a virulence factor of Acinetobacter baumannii: an emerging pathogen in critical care units A. J Curr Res Sci Med, 2017: 3:74–8.
Ryu SY, Baek W-K, Kim HA. Association of biofilm production with colonization among clinical isolates of Acinetobacter baumannii. Korean J Intern Med, 2017; 32:345–51.https://doi.org/10.3904/kjim.2015.287
Song JY, Cheong HJ, Noh JY, Kim WJ. In vitro comparison of anti-biofilm effects against carbapenem-resistant Acinetobacter baumannii: imipenem, colistin, tigecycline, rifampicin and combinations. Infect Chemother, 2015; 47:27–32.https://doi.org/10.3947/ic.2015.47.1.27
Thakre AD, Mulange SV, Kodgire SS, Zore GB, Mohan Karuppayil S. Effects of cinnamaldehyde, ocimene, camphene, curcumin and farnesene on Candida albicans. Adv Microbiol, 2016; 06:627–43.https://doi.org/10.4236/aim.2016.69062
Thummeepak R, Kongthai P, Leungtongkam U, Sitthisak S. Distribution of virulence genes involved in biofilm formation in multi-drug resistant Acinetobacter Baumannii clinical isolates. Int Microbiol, 2016; 19:121–9.

Article Metrics

849 Views 104 Downloads 953 Total

Year

Month

Related Search

By author names

Mohamed S H [PubMed] [Google Scholar ]

Salem D [PubMed] [Google Scholar ]

Azmy M [PubMed] [Google Scholar ]

Fam N S [PubMed] [Google Scholar ]

By article title