Research Article | Volume: 8, Issue: 11, November, 2018

Biocontrol potential of extracellular proteins from Xenorhabdus nematophilus on dengue vectors and the enhancement by response surface methodology

Vani Chandrapragasam Anu Jacob J. Christina Lydia Jayachandran   

Open Access   

Published:  Nov 30, 2018

DOI: 10.7324/JAPS.2018.81119
Abstract

Dengue fever is a prevalent and rapidly spreading disease. It is best controlled by controlling its vectors. Aedes aegypti and Aedes albopictus are the primary and secondary worldwide vectors, respectively, which are bred in peridomestic man-made water containers. Biological control is the most effective and sustainable method as there is no resurgence effect and does not harm humans. Our study includes the use of extracellular proteins of Xenorhabdus nematophilus, a Gram-negative bacterium widely used as biocontrol agents belonging to the family Enterobacteriaceae. The mortality rates of fourth instar larvae A. aegypti when treated with 250 μg of extracellular proteins of X. nematophilus, recorded to be 40% after 72 hours of exposure. The rate of mortality was observed minimum even at higher concentration. The optimization of the medium through response surface methodology showed that there was an increase in the production of extracellular proteins. These proteins played a very important role in the control of A. aegypti. The maximum rate of mortality was recorded to be 92% when treated with 200 μg of extracellular proteins within 48 hours of treatment. Our research proved that the media optimization enhanced the production of extracellular proteins in the X. nematophilus and it can be used as a biocontrol agent for the control of dengue vector.


Keyword:     Xenorhabdus nematophilus extracellular proteins dengue vector.


Citation:

Vani C, Jacob A, Christina Lydia J. Biocontrol potential of extracellular proteins from Xenorhabdus nematophilus on dengue vectors and the enhancement by response surface methodology. J App Pharm Sci, 2018; 8(11): 131–139.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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